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pET22b_pScaC-SNAP_6xHis

Plasmid

#228835

Purpose

E. coli expression of the SNAP-tagged ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

SNAPtag-TEV-6xHis

Expression

Bacterial

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTKDP-cat

Plasmid

#71321

Purpose

Donor plasmid - chloramphenicol resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMLC-BB1

Plasmid

#166922

Purpose

Backbone for assembly of multi-level gene expression controllers

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

CyOFP - Orange fluorescent protein - flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100 constitutive

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTHS

Plasmid

#166938

Purpose

Toehold switch multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- Toehold switch

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTKDP-hph

Plasmid

#71322

Purpose

Donor plasmid - hygromycin resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcSrc416

Plasmid

#17676

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y416F (SRC Chicken)

Expression

Mammalian

Mutation

Tyr 416 changed to Phe .

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pPtac

Plasmid

#166937

Purpose

Ptac single-level gene expression controller (SLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- PTAC

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDC

Plasmid

#166941

Purpose

Dual control based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Circuit expressing GFP- Dual Control

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pH-01

Plasmid

#166936

Purpose

Reverse Ptac promoter part (H)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

PTAC promoter - Block H- Flanked by BsaI restriction sites.

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-01

Plasmid

#166932

Purpose

Toehold switch part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Toehold Switch - Block G - Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pE-01

Plasmid

#166930

Purpose

Stop codon part (E)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

STOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-01

Plasmid

#166925

Purpose

sRNA part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

sRNA ToeHold - Block C- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pB-01

Plasmid

#166924

Purpose

Ptac promoter part (B)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

PTAC promoter - Block B- Flanked by BsaI restriction sites.

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pA-01

Plasmid

#166923

Purpose

LacI expression cassette (A)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

LacI expression cassette - Block A- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcR295

Plasmid

#17678

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R (SRC Chicken)

Expression

Mammalian

Mutation

Lys 295 changed to Arg.

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pSTC0

Plasmid

#39240

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ11m

Plasmid

#39245

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcR295/527

Plasmid

#17679

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Lys 295 changed to Arg. Tyr 527 changed to Phe.

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pVB004

Plasmid

#127188

Purpose

Tunable NOT gate without RiboJ insulator

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate and Reporter without RiboJ Insulator

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRAJ11

Plasmid

#39244

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12

Plasmid

#39246

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12m

Plasmid

#39247

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21

Plasmid

#39249

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21m

Plasmid

#39250

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSTC0r1

Plasmid

#39242

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

pLlacO-1+BBa_B0034+GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcSrc416/527

Plasmid

#17677

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y416F Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Tyr 416 changed to Phe . Tyr 527 changed to Phe .

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
SM425_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-KAN-DR

Plasmid

#244225

Purpose

Vector for golden gate cloning LbCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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SM426_pHAGE-CAG-mCherry-T2A-puro-DR-As_crRNA-KAN-DR

Plasmid

#244226

Purpose

Vector for golden gate cloning AsCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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SM271_EF1A-dHyperLbCas12a-4xSID-T2A-BlastR

Plasmid

#244209

Purpose

Mammalian expression of dHyperLbCas12a-4xSID with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PaPchE-AE

Plasmid

#242103

Purpose

Adenylase-epimerase didomain of the nonribosomal peptide synthetase PchE from Pseudomonas aeruginosa for the production of pyochelin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

PaPchE-AE

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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