Skip to main content
Addgene
Showing: 251 - 256 of 256 results

  1. gCOTS-pyl

    Plasmid
    #92048
    Purpose
    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Cyanobacteria s. elongatus genome recombination i…
    Tags
    Expression
    Mutation
    Promoter
    LeuP (native S. elongatus promoter) and PrcbL
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92048-plasmid-map-sequence-id-181707

    Enlarge

  2. pAM4787

    Plasmid
    #120088
    Purpose
    Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120088-plasmid-map-sequence-id-234398

    Enlarge

  3. pAM4788

    Plasmid
    #120089
    Purpose
    Cyanobacteria synthetic plasmid harboring the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS) to test the pSYSA-TA1 toxin–antitoxin cassette.
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    pAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120089-plasmid-map-sequence-id-234417

    Enlarge

  4. pAM5188

    Plasmid
    #120087
    Purpose
    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120087-plasmid-map-sequence-id-234428

    Enlarge

  5. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Expression
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92047-plasmid-map-sequence-id-194905

    Enlarge

  6. pRL838

    Plasmid
    #70680
    Purpose
    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.
    Depositor
    Peter Wolk
    Article
    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70680-plasmid-map-sequence-id-126382

    Enlarge

Showing: 251 - 256 of 256 results