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Plasmid#192959PurposeFor expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studiesDepositorInsertsptse5-CT
UseTagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A
Plasmid#128944Purposeexpresses R480A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAG324V
Plasmid#128947Purposeexpresses G324V mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeEH258A
Plasmid#129122Purposeexpresses H258A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeES157A
Plasmid#129121Purposeexpresses S157A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKTop::tse5-CT (1169-1281)
Plasmid#192955PurposeFor membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInserttse5-CT (1169-1281)
UseTagsExpressionBacterialMutationencodes for tse5 (1169-1281)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A-Q482A
Plasmid#128946Purposeexpresses R480A Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1229)
Plasmid#192948PurposeFor membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1229)
UseTagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1229)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::tse5-CT (1169-1300)
Plasmid#192956PurposeFor membrane insertion study; Expresses tse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInserttse5-CT (1169-1300)
UseTagsExpressionBacterialMutationencodes for tse5 (1169-1300)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::tse5-CT (1169-1317)
Plasmid#192957PurposeFor membrane insertion study; Expresses tse5-CT (1169-1317) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInserttse5-CT (1169-1317)
UseTagsExpressionBacterialMutationencodes for tse5 (1169-1317)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::tse5-CT (1169-1269)
Plasmid#192954PurposeFor membrane insertion study; Expresses tse5-CT (1169-1269) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInserttse5-CT (1169-1269)
UseTagsExpressionBacterialMutationencodes for tse5 (1169-1269)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::sptse5-CT_phoA-lacZalpha
Plasmid#192961PurposeTest the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putidaDepositorInsertsptse5-CT_phoA-lacZ_
UseTagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317) fused to phoA-lacZal…PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::tse5-CT (1169-1229)
Plasmid#192953PurposeFor membrane insertion study; Expresses tse5-CT (1169-1229) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInserttse5-CT (1169-1229)
UseTagsExpressionBacterialMutationencodes for tse5 (1169-1229)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1281)
Plasmid#192950PurposeFor membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1281)
UseTagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1281)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1269)
Plasmid#192949PurposeFor membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1269)
UseTagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1269)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1300)
Plasmid#192951PurposeFor membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1300)
UseTagsPelB signal peptideExpressionBacterialMutationencodes for sptse5 (1169-1300)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)
Plasmid#114100Purposefusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA5006
Plasmid#97016PurposeExpressing Pseudomonas aeruginosa orf PA5006 proteinDepositorInsertP aeruginosa orf PA5006
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA0856
Plasmid#97014PurposeExpressing Pseudomonas aeruginosa orf PA0856 proteinDepositorInsertP aeruginosa orf PA0856
UseTagsN-ter TEV cleavable 6HIsExpressionMutationN-terminal deletion (AA 1-34)PromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4511
Plasmid#97015PurposeExpressing Pseudomonas aeruginosa orf PA4511 proteinDepositorInsertP. aeruginosa orf PA4511
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only