We narrowed to 1,049 results for: IM

1. HP138-puro

   Plasmid

   #134246

   Purpose

   Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   Contains Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCB168

   Plasmid

   #37389

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   dynein cytoplasmic 1 intermediate chain 1 (DYNC1I1 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJM005

   Plasmid

   #134150

   Purpose

   Retroviral plasmid expressing GFP - Nde1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

   Insert

   Nde1_SSSC (NDE1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SSSC

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJM010

   Plasmid

   #134154

   Purpose

   Retroviral plasmid expressing GFP - Nde1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

   Insert

   Nde1_KMLL (NDE1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   KMLL

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTK88_GFP-Anillin

   Plasmid

   #46354

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   Anillin (ANLN Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   a1854 is changed to c to disrupt XhoI site withou…

   Promoter

   pBABE

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM808

   Plasmid

   #134181

   Purpose

   Lentiviral sgRNA plasmid with blasticidin resistance

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. H2B-mScarlet- hMeikin(1-332)-mNeonGreen

   Plasmid

   #174714

   Purpose

   Stable expression of fluorescent Separase cleavage sensor with human Meikin

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin
   

   Use

   Retroviral

   Tags

   H2B-mScarlet and mNeonGreen

   Expression

   Mammalian

   Mutation

   1-332

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTK47_mCherry-LGN-hardened

   Plasmid

   #46347

   Purpose

   rescues the phenotypes caused by LGN RNAi

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   LGN (GPSM2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

   Promoter

   CMV

   Available since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EcMBP165-cpGFP.PPYF.T203V.pRSET

   Plasmid

   #33373

   Purpose

   a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

   Depositor

   Loren Looger

   Article

   Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

   Insert

   Improved MBP based Maltose Biosensor
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted between AA1…

   Promoter

   T7

   Available since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZS220

    Plasmid

    #177642

    Purpose

    In vitro transcription of Patiria miniata Dishevelled

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Dishevelled
    

    Use

    In vitro transcription

    Tags

    GFP

    Expression

    Mutation

    K47A

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCB169

    Plasmid

    #37390

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    dynein light chain TcTex-type 3 (DYNLT3 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3xEGFP-mMeikin

    Plasmid

    #174708

    Purpose

    Mammalian expression of mouse Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin (Meikin Mouse)
    

    Use

    Tags

    3xEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTK162_mCherry-4.1G-hardened

    Plasmid

    #46361

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G (EPB41L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Modified for siRNA resistance to gcTccTcaTttCgaAc…

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTK38_mCherry-LGN-C

    Plasmid

    #46346

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    LGN C-terminal region (GPSM2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    contains aa365-684; deletion of S524

    Promoter

    CMV

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKM394

    Plasmid

    #134211

    Purpose

    Baculovirus expression of CENP-N and CENP-L

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-N-his-CENP-L
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKM410

    Plasmid

    #134212

    Purpose

    Baculovirus expression of CENP-T and CENP-W

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    GST-CENP-T+W
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKM463

    Plasmid

    #134214

    Purpose

    Baculovirus expression of CENP-U and CENP-Q

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    his-CENP-U + Q
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKM377

    Plasmid

    #134210

    Purpose

    Baculovirus expression of CENP-HKM and His-CENP-I

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-H/K/M untagged + his-I
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJM111

    Plasmid

    #134162

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_SSSC 295-C (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SSSC

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTK158_mCherry-4.1G-deltaCTD-hardened

    Plasmid

    #46360

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G (EPB41L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 886-1005, and modified for si…

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge