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pVAX-Help-SIINFEKL

Plasmid

#141350

Purpose

DNA vaccination plasmid containing SIINFEKL and several CD4+ T cell epitopes

Depositor

Ton Schumacher

Publication

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

HELP-SIINFEKL-KDEL

Expression

Mammalian

Promoter

CMV promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pF3-CAG-Tol-FRT

Plasmid

#141349

Purpose

Shuttling cassette containing the Tol ORF

Depositor

Ton Schumacher

Publication

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Tol cassette

Use

Mouse Targeting

Tags

HA

Expression

Mammalian

Promoter

CAG

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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PT2/C-fluc

Plasmid

#20203

Depositor

John Ohlfest

Publication

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

C-Fluc

Use

Transposon

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-A

Plasmid

#177811

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Hygro_RGECO1.2

Plasmid

#164592

Purpose

Stable integration and expression of RGECO1.2 in mammalian cells

Depositor

Joseph Hyser

Publication

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

R-GECO1.2

Use

Lentiviral

Expression

Mammalian

Mutation

R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA3_Gap43_Csx30_Cre

Plasmid

#197604

Purpose

Cre reporter for mammalian cells with Gap43 membrane anchor

Depositor

Feng Zhang

Publication

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Gap43_Csx30_Cre

Expression

Mammalian

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Chrm3_Csx30_Cre

Plasmid

#197603

Purpose

Cre reporter for mammalian cells with Chrm3 membrane anchor

Depositor

Feng Zhang

Publication

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Chrm3_Csx30_Cre

Expression

Mammalian

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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PABPC1

Plasmid

#155805

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PABPC1 (PABPC1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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IGF2BP1

Plasmid

#155676

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

IGF2BP1 (IGF2BP1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAF1-VP64-SeqFosA

Plasmid

#51430

Purpose

Artificial transcription factor with VP64 targeting c-Fos (TALE A)

Depositor

Xavier Darzacq

Publication

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosA

Expression

Mammalian

Promoter

CMV

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

Plasmid

#176538

Purpose

Mammalian expression of nuclear localized AwaIscB

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHS0663 pET45b(+) KraIscB-1 TwinStrep

Plasmid

#176536

Purpose

Bacterial expression of KraIscB-1-TwinStrep

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

KraIscB-1

Tags

TwinStrep

Expression

Bacterial

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHS0806 His14-bdSUMO-AwaIscB

Plasmid

#176539

Purpose

Bacterial expression of His14-bdSUMO-AwaIscB

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Use

CRISPR

Tags

14xHis-bdSUMO tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pENV8(GAAA)-GFPuv

Plasmid

#70128

Purpose

Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

Depositor

Robert Batey

Publication

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

tac

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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GAPDH

Plasmid

#155622

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

GAPDH (GAPDH Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL_miniUCOE-SFFVp-EGFP

Plasmid

#244972

Purpose

Express EGFP under miniUCOE-SFFV promoter. Xho I-miniUCOE-SFFVp-AgeI-EGFP.

Depositor

Xinnan Wang

Publication

Kwak et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

miniUCOE-SFFVp-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL_SFFVp-EGFP

Plasmid

#244971

Purpose

Express EGFP under SFFV promoter. Xho I-SFFVp-AgeI-EGFP.

Depositor

Xinnan Wang

Publication

Kwak et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

SFFVp-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_HA_CASP_sigma

Plasmid

#197614

Purpose

For expression of HA-tagged CASP sigma in E. coli

Depositor

Feng Zhang

Publication

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

HA-tagged CASP sigma

Expression

Bacterial

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAF1-VP16-SeqFosB

Plasmid

#51431

Purpose

Artificial transcription factor with VP16 targeting c-Fos (TALE B)

Depositor

Xavier Darzacq

Publication

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosB

Expression

Mammalian

Promoter

CMV

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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