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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Article

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMTL3

Plasmid

#127966

Purpose

constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

AAVS1-CAG-KRAB-dCas9-BFP-KRAB

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMTL5

Plasmid

#127967

Purpose

DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-99a-WT

Plasmid

#215880

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHC5

Plasmid

#85812

Purpose

To rescue the expression of DHHC5 in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 (Zdhhc5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHS

Plasmid

#85813

Purpose

To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 C134S mutant (Zdhhc5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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HT028_FCMV_CheRiff_EGFP

Plasmid

#208605

Purpose

Expressing CheRiff_EGFP under CMV promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

CheRiff_EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(5p)-WT

Plasmid

#215875

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(5p)-WT (MIR376A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(3p)-WT

Plasmid

#215876

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-WT (MIR376A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(5p)-WT

Plasmid

#215877

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-WT (MIR589 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-589(3p)-WT

Plasmid

#215878

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-WT (MIR589 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-376a2(3p)-WT

Plasmid

#215879

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-WT (MIR376A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-376a1(5p)-I-type

Plasmid

#215882

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of 376a1(5p)-I-type (MIR376A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-22(3p)-I-type

Plasmid

#215886

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-22(3p)-I-type (MIR22 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-376a2(3p)-I-type

Plasmid

#215887

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-99a-I-type

Plasmid

#215888

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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HT121_Fsyn_QuasAr6a(Q3_codon)_citrine

Plasmid

#208606

Purpose

Expressing QuasAr6a_Citrine with an alternate codon

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a

Use

Lentiviral

Tags

Citrine

Expression

Mutation

Promoter

hSyn

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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HT103_FCMV_QuasAr6a_Citrine

Plasmid

#178822

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6a carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a_Citrine

Use

Lentiviral

Tags

citrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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