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pTR-450 tNGFR-P2A-CXCR4

Plasmid

#186078

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a QTY

Plasmid

#162442

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ146a QTY

Plasmid

#162443

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a-sfGFP

Plasmid

#162446

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

truncation from aa52 to aa257

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pDONR223_CXCR4_WT

Plasmid

#81957

Purpose

Gateway Donor vector containing CXCR4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CXCR4 (CXCR4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Neuropeptide Y [L115/13R-2b]

Plasmid

#206639

Purpose

Mammalian Expression Plasmid of anti-Neuropeptide Y (Human). Derived from hybridoma L115/13-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Neuropeptide Y (Homo sapiens) recombinant Mouse monoclonal antibody (NPY Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(CysCNP)RS

Plasmid

#225054

Purpose

tRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al Chem 102385

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-E6AP Hect

Plasmid

#165102

Purpose

bacterial expression of a GST fusion of Human E6AP Hect domain

Depositor

Arthur Haas

Article

Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

Insert

E6AP Hect (UBE3A Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

E6AP Hect domain Amino acids 495-738 (5'QLNP…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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