Addgene

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pDONR221_SLCO3A1

Plasmid

#132293

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO3A1 (SLCO3A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLCO2B1_STOP

Plasmid

#161032

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO2B1 (SLCO2B1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC22A6_STOP

Plasmid

#161131

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A6 (SLC22A6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-SLC22A9_STOP

Plasmid

#161167

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A9 (SLC22A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-SLCO1B3_STOP

Plasmid

#161366

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1B3 (SLCO1B3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-SLCO3A1_STOP

Plasmid

#161459

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO3A1 (SLCO3A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
P. falciparum DHPS

Plasmid

#64080

Purpose

Expression of P. falciparum dihydropteroate synthase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

Insert

dihydropteroate synthase

Use

Tags

Histidine tag

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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S. aureus DHPS

Plasmid

#64079

Purpose

Expression of Staphylococcus aureus dihydropteroate synthase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

Insert

dihydropteroate synthase

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pHcRed-CLCa

Plasmid

#196911

Purpose

visualization of clathrin-coated vesicle in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

Clathrin-Coated light chain

Use

Tags

HcRed

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLCa

Plasmid

#196912

Purpose

Visualization of clathrin-coated pit formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

Insert

Clathrin-Coated light chain

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC22A10

Plasmid

#132046

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A10 (SLC22A10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLCO6A1

Plasmid

#132261

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO6A1 (SLCO6A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-SLC22A10_STOP

Plasmid

#161212

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A10 (SLC22A10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-SLCO6A1_STOP

Plasmid

#161427

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO6A1 (SLCO6A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET28b-LipB

Plasmid

#30165

Depositor

SeWon Suh

Article

Kim do et al Proteins. 2008 Mar . 70(4):1620-5.

Insert

lipoate protein ligase B

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pXM03

Plasmid

#121150

Purpose

This plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coli

Depositor

Minjuan Xu

Article

He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

Insert

XimB, IDS2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PP7_mCherry

Plasmid

#61763

Purpose

Expresses PP7 coat protein fused to mCherry

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

PP7 coat protein fused to mCherry

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Ubc promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Delta DED FLIP-L

Plasmid

#13790

Depositor

Guy Salvesen

Article

Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

Insert

DELTA DED FLIP LONG (CFLAR Human)

Use

Tags

8 x His tag

Expression

Bacterial

Mutation

Delta DED removes first 186 residues

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkaš et al Engineering Microbiology, 2022

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEAST_J23101-bphD

Plasmid

#128138

Purpose

The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

bphD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Availability

Academic Institutions and Nonprofits only

Enlarge