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Plasmid#226561PurposeExpress H2B-GFP in zebrafish.DepositorInserth2b (h2bc1 Zebrafish)
UseSynthetic Biology; Zebrafish expressionTagsEGFPExpressionMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_4ACJ
Plasmid#234212PurposeHeterologous protein expression of Wufb25h12 in Escherichia coliDepositorInsert4ACJ (oxr1a Zebrafish)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1KQX
Plasmid#234181PurposeHeterologous protein expression of cellular retinol-binding protein type II in Escherichia coliDepositorInsert1KQX (rbp2a Zebrafish)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-CMV-zdgat2FLAG-SV40
Plasmid#232141PurposeExpresses zebrafish dgat2 with a C-terminal 3xFLAG tag under control of the CMV promoterDepositorInsertdgat2 (dgat2 Zebrafish)
UseZebrafish expressionTags3xFLAGExpressionMutationPromoterCMVAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ dgat1a-FLAG
Plasmid#232143PurposeExpresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoterDepositorInsertdgat1a (dgat1a Zebrafish)
UseZebrafish expressionTagsFLAGExpressionMutationPromoterCMVAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ mogat3b-FLAG
Plasmid#232145PurposeExpresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoterDepositorInsertmogat3b
UseZebrafish expressionTagsFLAGExpressionMutationPromoterCMVAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-Crestin-pro (JDW 1320)
Plasmid#229830PurposeA gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expressionDepositorInsertCrestin promoter, c-fos minimal promoter, and b-globin intron
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry
Plasmid#230043PurposePartial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.DepositorInsertlox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoterfabp10aAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-zdgat2-CDS-FLAG
Plasmid#232142PurposeGateway middle entry vector containing zebrafish dgat2 with a C-terminal 3xFLAG tagDepositorInsertdgat2 (dgat2 Zebrafish)
UseGateway cloning middle entryTags3xFLAGExpressionMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-mogat3b in situ probe
Plasmid#232136Purposezebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymeraseDepositorInsertmogat3b
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcRII-mogat3a in situ probe
Plasmid#232135Purposezebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertmogat3a
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-dgat1a in situ probe
Plasmid#232137Purposezebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertdgat1a (dgat1a Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-dgat1b in situ probe
Plasmid#232138Purposezebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertdgat1b
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-mogat2 in situ probe
Plasmid#232139Purposezebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertmogat2 (mogat2 Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-dgat2 in situ probe
Plasmid#232134Purposezebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymeraseDepositorInsertdgat2 (dgat2 Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0_shRNAHsCavin1_Del4UC1_EGFP
Plasmid#229691PurposeLentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)DepositorUseLentiviralTagsEGFPExpressionMutationDel4UC1PromoterLTR viral promoterAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0_shRNAHsCavin1_DrCavin1b_EGFP
Plasmid#229690PurposeLentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish Cavin1 (Cavin1 rescue)DepositorUseLentiviralTagsEGFPExpressionMutationPromoterLTR viral promoterAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-RR-zrif1_exon8
Plasmid#194434PurposeRight (RR) TALEN for mutating zebrafish rif1 geneDepositorInsertzrif1_ex8_RIGHT (rif1 Zebrafish)
UseTALENTagsExpressionMutationPromoterCMV IE94Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-DD-zrif1_exon8
Plasmid#194435PurposeLeft (DD) TALEN for mutating zebrafish rif1 geneDepositorInsertzrif1_ex8_LEFT (rif1 Zebrafish)
UseTALENTagsExpressionMutationPromoterCMV IE94Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GATA1
Plasmid#194436PurposeIn Situ hybridization probesDepositorInsertGATA1a (gata1a Zebrafish)
UseIn situ hybridizationTagsExpressionMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only