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pSuperCRISPR-sgRNA-RL714-TTTA

Plasmid

#215867

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL714 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL864-TTTA

Plasmid

#215859

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL864 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL864R-TTTA

Plasmid

#215861

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL864R with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL554-TTTA

Plasmid

#215863

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL554 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag KO sgRNA

Plasmid

#207102

Purpose

pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTCGATGTTGGTCCGCGCGA

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #2

Plasmid

#207106

Purpose

pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CTGAAGGAACAGACTAATTC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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53BP1 KO sgRNA

Plasmid

#207104

Purpose

pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGATTCTCAGCCTGAAAGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPIBY55

Plasmid

#213706

Purpose

Express the affinity sorting tag TST-EGFP- GPIBY55.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPICEAM7

Plasmid

#213705

Purpose

Express the affinity sorting tag TST-EGFP- GPICEAM7.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPIDAF

Plasmid

#213704

Purpose

Express the affinity sorting tag TST-EGFP- GPIDAF.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TREER

Plasmid

#208321

Purpose

Bacterial expression of the His-tagged peptide TREER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_RB-TnV2_pJEx

Plasmid

#213908

Purpose

Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-EGFP-Cre

Plasmid

#207132

Purpose

Lentiviral vector to express EGFP-Cre

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

EGFP-Cre

Use

Lentiviral

Promoter

SSFV

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT346

Plasmid

#204505

Purpose

5S rDNA used as a probe for chromosome FISH in nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

5S rDNA (C. elegans)

Use

Other

Promoter

T7

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-pnRFP

Plasmid

#201679

Purpose

Expresses pnRFP in mammalian cells

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

pnRFP

Expression

Mammalian

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh2-mRFP

Plasmid

#170982

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 LvWSCP-EGFP

Plasmid

#196480

Purpose

Mammalian expression of WSCP-EGFP

Depositor

Fei Sun

Article

Li et al Nat Commun. 2022 Jun 9;13(1):3197. doi:

Insert

LvWSCP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-SpDcp2opt_1-242_AD

Plasmid

#148547

Purpose

Bacterial Expression of SpDcp2opt_1-242

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242 (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpDCP1_X

Plasmid

#148003

Purpose

Bacterial Expression of SpDCP1

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP1 (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-CtDCP2_1-267_X

Plasmid

#148004

Purpose

Bacterial Expression of CtDCP2_1-267

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP2_1-267

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C_1248-1600_I

Plasmid

#146590

Purpose

Mammalian Expression of HsTNRC6C_1248-1600

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1248-1600 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C_1248-1690-delDUF_I

Plasmid

#146591

Purpose

Mammalian Expression of HsTNRC6C_1248-1690-delDUF

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1248-1690-delDUF (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C_1248-1690-delDUFMID2Cter_I

Plasmid

#146592

Purpose

Mammalian Expression of HsTNRC6C_1248-1690-delDUFMID2Cter

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1248-1690-delDUFMID2Cter (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C_1248-1690-delMID1_I

Plasmid

#146594

Purpose

Mammalian Expression of HsTNRC6C_1248-1690-delMID1

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1248-1690-delMID1 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C_1248-1690-delMID2_I

Plasmid

#146595

Purpose

Mammalian Expression of HsTNRC6C_1248-1690-delMID2

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1248-1690-delMID2 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C_1248-1690-delRRM_I

Plasmid

#146596

Purpose

Mammalian Expression of HsTNRC6C_1248-1690-delRRM

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1248-1690-delRRM (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C_1248-1690_H

Plasmid

#146512

Purpose

Mammalian Expression of HsTNRC6C_1248-1690

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1248-1690 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C-DUF-RRM_H

Plasmid

#146515

Purpose

Mammalian Expression of HsTNRC6C-DUF-RRM

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C-DUF-RRM (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C-MID2_H

Plasmid

#146517

Purpose

Mammalian Expression of HsTNRC6C-MID2

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C-MID2 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C-MID2-Cterm_H

Plasmid

#146518

Purpose

Mammalian Expression of HsTNRC6C-MID2-Cterm

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C-MID2-Cterm (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C-MID2-RRM_H

Plasmid

#146519

Purpose

Mammalian Expression of HsTNRC6C-MID2-RRM

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C-MID2-RRM (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C-delAGOhook_F

Plasmid

#146307

Purpose

Mammalian Expression of HsTNRC6C-delAGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delAGOhook (TNRC6C Human)

Expression

Mammalian

Mutation

1 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C-delMI_F

Plasmid

#146308

Purpose

Mammalian Expression of HsTNRC6C-delMI

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C-delMII_F

Plasmid

#146310

Purpose

Mammalian Expression of HsTNRC6C-delMII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMII (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6C-delMI-MII_F

Plasmid

#146311

Purpose

Mammalian Expression of HsTNRC6C-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI-MII (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-RLuc-6xbsMS2-8xlet7_AC

Plasmid

#148433

Purpose

Mammalian Expression of 6xbsMS2-8xlet7

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-8xlet7

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-TISU-RLuc-6xbsMS2_AC

Plasmid

#148434

Purpose

Mammalian Expression of 6xbsMS2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-TISU-RLuc-6xbsMS2-8xlet7_AC

Plasmid

#148435

Purpose

Mammalian Expression of 6xbsMS2-8xlet7

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-8xlet7

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-TISU-RLuc-6xbsMS2-random_AC

Plasmid

#148437

Purpose

Mammalian Expression of 6xbsMS2-random

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-random

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-no5UTR-lambdaN-HA-GST-V5His6_AC

Plasmid

#148405

Purpose

Insect Expression vector

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Type

Empty backbone

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1C-no5UTR-Fluc-5BoxB_AC

Plasmid

#148408

Purpose

Insect Expression of 5BoxB

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

5BoxB

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

Plasmid

#185679

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HiBiT-SMO

Plasmid

#195851

Purpose

Expresses mouse SMO with an N-terminal HiBiT; contains D54N and R690G

Depositor

Gunnar Schulte

Article

Turku et al Nat Commun. 2021 Jun 24;12(1):3919. doi:

Insert

SMO (Smo Mouse)

Tags

HiBiT

Expression

Mammalian

Mutation

D54N and R690G

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpG-SpDcp1opt_AD

Plasmid

#148538

Purpose

Bacterial Expression of SpDcp1opt

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR-U6-gRNA-TRE3G-Nluc

Plasmid

#192659

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, TRE3G

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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