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pYSD_lambda_mRFP

Plasmid

#217831

Purpose

Yeast surface display Fab vector with lambda light chain and mRFP

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD_kappa_mRFP

Plasmid

#217830

Purpose

Yeast surface display Fab vector with kappa light chain and mRFP

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMMP_lambda

Plasmid

#217829

Purpose

Mini-mutagenesis plasmid for lambda light chain YSD Fabs

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMMP_kappa

Plasmid

#217828

Purpose

Mini-mutagenesis plasmid for kappa light chain YSD Fabs

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt7

Plasmid

#213630

Purpose

Bacterial expression of SUMO-tagged mouse FL-syt7

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt7 (Syt7 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

Plasmid

#201964

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E162A,M164A,R167A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

Plasmid

#201965

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E198A, R199A,E200A, D201A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

Plasmid

#201967

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R232A, K233A, R234A, K235A, R236A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

Plasmid

#201968

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

W255A,N262E,K266E

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt6

Plasmid

#184507

Purpose

Mammalian expression of Synaptotagmin 6 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 6 (Syt6 Mouse)

Use

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETmsGFPSyt1(80 JuxtaK-142)

Plasmid

#209013

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(80 JuxtaK-421)

Plasmid

#209008

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209016

Purpose

Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209011

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A D197,199N

Plasmid

#195704

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9 with D1…

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B D330, 332N

Plasmid

#195705

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9 with D3…

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-cUnaG

Plasmid

#207631

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-cUnaG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-LPETG

Plasmid

#207629

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-LPETG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C ACT-DCC-Halo

Plasmid

#195148

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove act…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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