Addgene

We narrowed to 158,104 results for: Gene

Showing: 1181 - 1200 of 158104 results

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pCRISPaint-2A-TurboGFP-PEST

Plasmid

#67180

Purpose

CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

T2A-TurboGFP-PEST

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pG4

Plasmid

#162605

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pYM70

Plasmid

#119922

Purpose

CaHygB plasmids that facilitate genetic manipulations in the fungal pathogen

Depositor

Brian Wong

Article

Basso LR et al Yeast. 2010 Dec;27(12):1039-48. doi: 10.

Insert

CaHygB

Use

Tags

CaHygB

Expression

Mutation

Promoter

TEF2

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDae069 (eMutaT7[PmCDA1] with optimized RBS)

Plasmid

#187621

Purpose

Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

PmCDA1-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pG2

Plasmid

#162603

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKM427

Plasmid

#210788

Purpose

L5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transfer

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2021;2314:301-321. doi

Insert

Defective hygromycin resistant gene

Use

Tags

Expression

Bacterial

Mutation

Changed hygromycin resistant gene W190 codon (TGG…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAsp-AMA-gpdA-ptr

Plasmid

#191016

Purpose

CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)

Depositor

Perng-Kuang Chang

Article

Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

Type

Empty backbone

Use

Unspecified; Aspergillus

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDusk

Plasmid

#43795

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pG1

Plasmid

#162602

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDae029 (eMutaT7[TadA-8e])

Plasmid

#187620

Purpose

Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

TadA-8e-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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MSGV Hu Acceptor

Plasmid

#64269

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

none

Expression

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(272)

Plasmid

#44375

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(272) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(272)

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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mARG1 cassette 1

Plasmid

#134343

Purpose

Mammalian acoustic reporter gene 1 cassette 1 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 1

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 2

Plasmid

#134344

Purpose

Mammalian acoustic reporter gene 1 cassette 2 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 3

Plasmid

#134345

Purpose

Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 3

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJS169

Plasmid

#48882

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Insert

lacI with ssrA tag

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Mutation

Promoter

Para/lac

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pG3

Plasmid

#162604

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge