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pET28a(+)-NEDD4L_W3-W4-HECT_C874only

Plasmid

#187711

Purpose

Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 with mutations (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491; mutations C702A, C776…

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_FL-CS

Plasmid

#187713

Purpose

Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_Δ1,2-linker

Plasmid

#187714

Purpose

Express GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 226-382 removed (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 226-382

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_Δ2,3-linker

Plasmid

#187715

Purpose

Express GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 418-495 removed (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 418-495

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)

Plasmid

#187716

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues XX (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-347

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-Clover

Plasmid

#126572

Purpose

Multisite gateway vector for 3' tagging with Clover. Parton lab clone DJZ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Clover

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-Bin1a

Plasmid

#176020

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Use

Leishmania cell free expression

Tags

EGFP

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-Bin1a

Plasmid

#176024

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1a. Parton lab clone GSS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Use

Leishmania cell free expression

Tags

mCherry

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMIR-31-192-193a-Reporter

Plasmid

#71874

Purpose

Mammalian expression vector for the analysis of miR-31-19α-193a activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Binding sequence targeted by miR-31, miR192, and miR-193a-5p

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

Plasmid

#85452

Purpose

Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

Depositor

Aviv Regev

Article

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1

Plasmid

#121478

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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dCas12Max-ABE

Plasmid

#195335

Purpose

vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized dCas12Max-ABE

Expression

Mammalian

Mutation

N243R, E336R, D1049A

Promoter

CBh, CMV, hU6

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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hfCas12Max

Plasmid

#195336

Purpose

vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized hfCas12Max

Tags

3xFlag

Expression

Mammalian

Mutation

N243R, E336R, D892R

Promoter

CBh, CMV, hU6

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PD-L1 3SA

Plasmid

#121493

Purpose

Expression of human PD-L1 3SA in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1 (CD274 Human)

Tags

HA

Expression

Mammalian

Mutation

S176A, T180A, S184A

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PD-L1

Plasmid

#121492

Purpose

Expression of human PD-L1 WT in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1 (CD274 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX/PD-L1 WT

Plasmid

#121466

Purpose

Expression of GST-human PD-L1 in E. Coli

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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dCas12Max-CBE

Plasmid

#195337

Purpose

vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized dCas12Max-CBE

Expression

Mammalian

Mutation

N243R, E336R, D1049A

Promoter

CBh, CMV, hU6

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-sgCTL

Plasmid

#234771

Purpose

Non Targeting Control

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Non Targeting Control sgRNA

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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PDGFRalpha-D842V #1

Plasmid

#201407

Purpose

Inserts PDGFRalpha-D842V to activate the MAP kinase pathway.

Depositor

Maria Castro

Article

Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

Insert

PDGFRalpha-D842V (Pdgfra Mouse)

Expression

Mammalian

Mutation

D842V

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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