We narrowed to 1,621 results for: nif

1. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPP537

   Plasmid

   #158803

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Use

   Tags

   2xSV40NLS-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. His-CL7-2NLS-SpyCas9(dS)-A22p3star

   Plasmid

   #222970

   Purpose

   Bacterial expression of A22p-fused SpyCas9 construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His-CL7-2NLS-SpyCas9(dS)-A22p3
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MCP/PCP RNA Lantern

   Plasmid

   #226095

   Purpose

   RNA tracking

   Depositor

   Jennifer Prescher

   Article

   Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

   Insert

   NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Halo-GPI-RUSH

   Plasmid

   #166903

   Purpose

   RUSH cargo: Str-KDEL_IRES_SBP-Halo-GPI

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Fra-2

   Plasmid

   #187907

   Purpose

   Expresses Fra-2 in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   Fra2 (Fosl2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Sar1b-mOrange2

   Plasmid

   #166899

   Purpose

   mammalian expression of Sar1b tagged with mOrange2

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   mOrange2
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Halo-TNFa-RUSH

   Plasmid

   #166901

   Purpose

   RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-Halo

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMJ841

   Plasmid

   #39318

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   T7

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p2CT-His-MBP-Lse_C2c2_WT

    Plasmid

    #83486

    Purpose

    Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lse_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mApple-TNFa-RUSH

    Plasmid

    #166902

    Purpose

    RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p2CT-His-MBP-Lsh_C2c2_WT

    Plasmid

    #83487

    Purpose

    Bacterial expression of WT L. shahii C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lsh_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMJ825

    Plasmid

    #39315

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sar1b-YFP

    Plasmid

    #166897

    Purpose

    mammalian expression of Sar1b tagged with YFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    Sar1b
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mApple-TfR-RUSH

    Plasmid

    #166906

    Purpose

    RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mApple

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SarI T39N YFP

    Plasmid

    #128155

    Purpose

    Expression of Sar1 with T39N dominant negative mutation

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    Sar1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    T39N

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPP381

    Plasmid

    #158798

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RuvC-inactivating mutation D394A

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Halo-TfR-RUSH

    Plasmid

    #166905

    Purpose

    RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

    Plasmid

    #222991

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
    

    Use

    CRISPR

    Tags

    CL7, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge