We narrowed to 1,843 results for: rigi

1. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

   Plasmid

   #117070

   Purpose

   Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

   Depositor

   Kim Failor

   Inserts

   Cas9
   SacB
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pconst #1-RFP

   Plasmid

   #81075

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23119

   Available Since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAL1

   Plasmid

   #197285

   Purpose

   Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

   Depositor

   Bogumil Karas

   Article

   Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

   Insert

   Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GTT-g

   Plasmid

   #81109

   Purpose

   Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   sfGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. GTH-r

   Plasmid

   #81106

   Purpose

   Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   mCherry
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPpT4- TEF2prom-TIR1F74G-FLAG

   Plasmid

   #189727

   Purpose

   Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   TEF2

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nup54 no FG-mEGFP494(0)

   Plasmid

   #164269

   Purpose

   Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP

   Depositor

   Sanford Simon

   Article

   Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

   Insert

   Nup54 (NUP54 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   deleted the FG region of Nup54

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH652-RLuc/maxFLuc

   Plasmid

   #29698

   Depositor

   Tobias von der Haar

   Article

   Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

   Insert

   Firefly Luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB3aZ_14*

   Plasmid

   #98542

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Michael Sauer, Brigitte Gasser, Diethard Mattanovich

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CTE-Luc

    Plasmid

    #60881

    Purpose

    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

    Depositor

    Vinay Nandicoori

    Article

    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

    Insert

    Luc-CTE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJUMP27ts[mCherry]-1A(lacZ)

    Plasmid

    #126980

    Purpose

    Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    RepA A56V thermosensitive mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. THI VP64 pGAP T5

    Plasmid

    #161492

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. THI VP64 pGAP T1

    Plasmid

    #161488

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCK300

    Plasmid

    #87766

    Purpose

    empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PacrAB-acrAB-rfp + PmutS-mutS-yfp

    Plasmid

    #121443

    Purpose

    plasmid expressing translational fusions of AcrAB and MutS

    Depositor

    Mary Dunlop

    Article

    El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

    Inserts

    PmutS-mutS-yfp
    PacrAB-acrAB-rfp
    

    Use

    Synthetic Biology; Pbb vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PacrAB and PmutS

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. BB3aN_14*

    Plasmid

    #98548

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GTH-g

    Plasmid

    #81104

    Purpose

    Gene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB2_EF

    Plasmid

    #98538

    Purpose

    empty BB2 for transcription unit cloning

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM532

    Plasmid

    #112529

    Purpose

    pZA1D-hrtR - HrtR expressed consitutively from promoter ProD with RBS2, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Insert

    HrtR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProD

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. GTL-g

    Plasmid

    #81099

    Purpose

    Gene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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