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pCellFree_G03 STAT6

Plasmid

#67123

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT6 (STAT6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP3

Plasmid

#67083

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP3 (TFDP3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP1

Plasmid

#67084

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP1 (TFDP1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP2

Plasmid

#67088

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP2 (TFDP2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pPK700

Plasmid

#38146

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

let-60p::ptc-3::gfp (ptc-3 Nematode)

Tags

EGFP

Expression

Worm

Promoter

let-60

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP Δcen mCherry

Plasmid

#45227

Depositor

Michael Lampson, Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

vsv Cenp-B INCENP Δcen mCherry

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
NLS-ABE8e-GFP

Plasmid

#251972

Purpose

Yeast nDNA editing with ABE8e

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE8e

Use

CRISPR

Tags

3xNLS-3xFLAG-EGFP and NLS

Expression

Yeast

Promoter

GPD

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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LIMK1-s

Plasmid

#245963

Purpose

Expression of inactive LIMK-1 with some dominant negative acivity and GFP from separate CMV promoter

Depositor

James Bamburg

Insert

LIM domain kinase 1 (Limk2 Mouse)

Expression

Mammalian

Mutation

deletion of 20 amino acids from kinase domain

Promoter

CMV for LIMK and separate CMV for eGFP

Available Since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-puro_TRE3VG_mCherry-Responder

Plasmid

#230052

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of mCherry through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

mCherry

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-sncb-KALTA4

Plasmid

#239986

Purpose

Expresses GAL4 under control of the sncb promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and sncb

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-stmn1b-KALTA4

Plasmid

#239987

Purpose

Expresses GAL4 under control of the stmn1b promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and stmn1b

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-cytoFLARE1.1-TEV

Plasmid

#234518

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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YX022 (PB)EF1a-LIDARsensor (hygR)

Plasmid

#233431

Purpose

EF1a-driven LIDAR reporter RNA

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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dimerTomato-wGBD

Plasmid

#129635

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

Cdc42 binding domain of N-WASP

Expression

Mammalian

Promoter

CMVdel

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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PAF23

Plasmid

#223171

Purpose

Expresses yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Article

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

green fluorescent protein

Expression

Yeast

Promoter

Saccharomyces cerevisiae CTR1

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-giantin-ATG_donor

Plasmid

#222316

Purpose

Perform knock-in by homologous recombination in giantin/GOLGB1 gene.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

EGFP and homology arms for giantin/GOLGB1

Use

CRISPR

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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apt-CBE-SL3

Plasmid

#219940

Purpose

Mammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_16-aa_evo-rA1_2xUGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2-TLCV2

Plasmid

#196331

Purpose

the TLCV2 plasmid with Tol2 recombination sites for integration

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

Cas9-2A-eGFP

Use

CRISPR; Fish expression

Expression

Bacterial, Mammalian, and Y…

Promoter

Tight TRE promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGH1110

Plasmid

#223682

Purpose

U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-C-GFP-NLS

Plasmid

#222604

Purpose

Expression of MAC3-tagged GFP-NLS (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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