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GLY, Linear-3, PolyU(4)

Plasmid

#166989

Purpose

Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(4)

Plasmid

#166985

Purpose

Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt

Plasmid

#166995

Purpose

Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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GP826

Plasmid

#164551

Purpose

Expresses Smo-FLAG K444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GP825

Plasmid

#164550

Purpose

Expresses Smo-FLAG K434, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GP824

Plasmid

#164549

Purpose

Expresses Smo-FLAG K434, 444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PD30

Plasmid

#164543

Purpose

Expresses Drd1-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP807

Plasmid

#164535

Purpose

Expresses Smo noK-FLAG-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG_Ub noK

Mutation

All 21 cytoplasmic Lysines of Smoothened and all…

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2365 - Intron GG1 - 250bp no PATC

Plasmid

#159883

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2345 - Intron GG2 - 250bp no PATC

Plasmid

#159884

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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