We narrowed to 10,134 results for: Coli

#162681

Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#169160

To express nsp14-nsp10 fusion protein in E. coli

John Diffley

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

July 6, 2021

Academic Institutions and Nonprofits only

#162682

Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 6, 2021

Academic Institutions and Nonprofits only

#233276

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

Mohan Babu, Andrew Emili

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

March 17, 2025

Academic Institutions and Nonprofits only

#233263

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

Mohan Babu, Andrew Emili

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

March 17, 2025

Academic Institutions and Nonprofits only

#162673

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162683

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162686

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162687

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#227238

Inducible ATP sensor for E. coli

Fuzhong Zhang

Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

Jan. 17, 2025

Academic Institutions and Nonprofits only