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Plasmid#194437PurposeIn Situ hybridization probesDepositorInsertβ-globin
UseIn situ hybridizationTagsExpressionMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430-G-G mm
Plasmid#221728PurposeExpression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.DepositorInsertBleoR-miR-430-G-G mm
UseFish expressionTagsExpressionMutationG-G mismatch of miR-430 sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430-10-11 mm
Plasmid#221727PurposeExpression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.DepositorInsertBleoR-miR-430-10-11 mm
UseFish expressionTagsExpressionMutationMismatch at positions 10 and 11 of miR-430 sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430
Plasmid#221726PurposeExpression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.DepositorInsertBleoR-miR-430
UseFish expressionTagsExpressionMutationPromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsN-drAgo2
Plasmid#221717PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.DepositorInserthsN-drAgo2 (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationSwap of N-domain for human Ago2 N-domainPromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-A
Plasmid#221713PurposeExpression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-A (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD683A in active sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA
Plasmid#227773PurposeExpression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafishDepositorInsertsCas9-t2A-GFP
4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterU6 and tp1Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP
Plasmid#227774PurposeMiddle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloningDepositorInsertloxP-dsR-loxP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p103DestTol2pA2-4xU6sgRNA
Plasmid#227778PurposeDestination vector with U6 driven 4 sgRNAsDepositorInsert4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA
Plasmid#226834PurposeExpression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafishDepositorInsertsCas9-t2A-GFP
4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterOlactb and U6Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol1 - 14xUAS:CaSR-EGFP
Plasmid#191502PurposeA UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.DepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol1 transgenes…TagsExpressionMutationPromoter14X UASAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hcar1-4-mKate2
Plasmid#226712Purposeoverexpression red fluorescent hcar1-4 protein in zebrafish larvaeDepositorInserthcar1-4
UseUnspecified; Zebrafish expressionTagsmKate2ExpressionMutationPromoterSP6Available sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P--Dr-aCateninFL
Plasmid#191740PurposeExpresses FL D rerio catenin in bacteriaDepositorInsertCTNNA1 (ctnna1 Zebrafish)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-5.0dand5:EGFP
Plasmid#223327PurposeExpresses EGFP in Kupffer's vesicle cells in zebrafish embryosDepositorInsert5.0kb dand5 promoter (dand5 Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2D-A
Plasmid#221734PurposeExpression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2D-A (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationD683A in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2DY-EF
Plasmid#221733PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationD651E and Y680F in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2Y-F
Plasmid#221732PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationY680F in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2D-E
Plasmid#221731PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2D-E (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationD651E in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2
Plasmid#221730PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2 (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2DY-EF
Plasmid#221716PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD651E and Y680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only