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pECE M2-SH3PXD2A WT

Plasmid

#69813

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-140)-2xFv-flag

Plasmid

#106074

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-140)-2xFv-flag (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

flag

Expression

Mammalian

Mutation

amino acids 1-140

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.KAT5

Plasmid

#184647

Purpose

Retroviral expression of mouse Kat5

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Kat5 (Kat5 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-182)-2xFv-Venus

Plasmid

#106077

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-182)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-182

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-182)-2xFv-flag

Plasmid

#106075

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-182)-2xFv-flag (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

flag

Expression

Mammalian

Mutation

amino acids 1-182

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

Plasmid

#226350

Purpose

Expression of proteasome substrate with tail for molecular ruler

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

titin (TTN Human)

Use

Tags

Expression

Bacterial

Mutation

V15P

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pZW16 (CK2alpha')

Plasmid

#27087

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-MYC

Plasmid

#130695

Purpose

Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus-Plasma

Plasmid

#106081

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

hMLKL-Venus-Plasma (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

plasma membrane-targetting domain of H-Ras (aa 1-…

Expression

Mammalian

Mutation

Promoter

dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PQBP1

Plasmid

#25804

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Apr 21. ():.

Insert

PQBP1 (PQBP1 Human)

Use

Tags

6xHA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pGEMHE-mClover3-MAP4-MTBD

Plasmid

#171498

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

So et al Science. 2019 Jun 28;364(6447). pii: 364

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mClover3

Expression

Mutation

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Article

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-WW mutant

Plasmid

#17792

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-140)-2xFv-Venus

Plasmid

#106076

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-140)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-140

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFPc3-Neo

Plasmid

#189741

Purpose

The construct was used to express GFP in mammalian cells as the control for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-SOX2

Plasmid

#130693

Purpose

Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human SOX2 (SOX2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-BASIC-ARHGAP29 PROMOTER

Plasmid

#104155

Purpose

luciferase reporter with ARHGAP29 promoter

Depositor

Marius Sudol

Article

Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

Insert

ARHGAP29 promoter (ARHGAP29 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

ARHGAP29

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2-S127A

Plasmid

#17794

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pGP18 (CK2alpha' K69M)

Plasmid

#27090

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' K69M (CSNK2A2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K69M

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pLVX-Flag-Polß(WT)-IRES-puro

Plasmid

#128653

Purpose

mammalian expression of Flag-Polß(WT) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

Plasmid

#176839

Purpose

To recruit SAM complex to human CYP3A4 proximal enhancer.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer sgRNA for activation

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAB07 (CK2beta)

Plasmid

#27091

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

Plasmid

#128010

Purpose

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Use

Drosophila osc reporter construct

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus-Mito

Plasmid

#106080

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

hMLKL-Venus-Mito (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

mitochondrial-targetting domain of Bcl-xl

Expression

Mammalian

Mutation

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(D256A)-IRES-puro

Plasmid

#128677

Purpose

mammalian expression of Flag-Polß(D256A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D256A

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC-MYC.Mm.KAT5

Plasmid

#184652

Purpose

Retroviral expression of mouse Kat5

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Kat5 (Kat5 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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D28 Cortactin GFP

Plasmid

#26723

Depositor

Anna Huttenlocher

Article

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion removed 28 amino acids (aa# 334-361) enc…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA 3.2/V5-DEST SNAP29-V5

Plasmid

#69821

Purpose

Expresses SNAP29-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Synaptosomal-associated protein 29 (SNAP29 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

cmv

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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