Addgene

We narrowed to 11,041 results for: Hal

Showing: 2401 - 2440 of 11041 results

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pGGA-pCLV3

Plasmid

#210528

Purpose

Cloning A. thaliana CLV3 promoter as GreenGate module A

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pCLV3 (CLV3 Mustard Weed)

Use

Synthetic Biology; Golden gate compatible cloning…

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB560-NADK2

Plasmid

#203760

Purpose

For Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

NAD KINASE2

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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NbV5-TEV219S153N

Plasmid

#201488

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-TEV219S153N

Use

Tags

TEV219 S153N

Expression

Mammalian

Mutation

S153N in TEV219

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1176

Plasmid

#202774

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-345

Plasmid

#195456

Purpose

Lentiviral Integratable, constitutive expression of synZiFTR: ZF1-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - 3XFLAG - NLS - ZF1 - p65

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-346

Plasmid

#195457

Purpose

Lentiviral Integratable, constitutive expression of synZiFTR: ZF3-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - 3XFLAG - NLS - ZF1 - p65

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-441

Plasmid

#195465

Purpose

Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: HP1a-ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - HP1a - ZF3 - ERT2 - SMASh

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET15 PemrR-gfp biosensor

Plasmid

#185793

Purpose

The pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.

Depositor

Steven Hallam

Article

Ho et al ACS Synth Biol. 2018 Feb 16;7(2):392-398

Insert

pEmrR-gfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

PemrR

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_SEAP

Plasmid

#154461

Purpose

Expression of SEAP and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

SEAP

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0131

Plasmid

#172793

Purpose

Level 0 module containing CDS2ns_deltamPTAC5

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature PTAC5 with C-ter delection (41-252), Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8_Cap

Plasmid

#175797

Purpose

de novo thick circular tandem repeat protein capped to prevent multimerization

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8_Cap

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SH2-free

Plasmid

#175800

Purpose

Human Nck2 SH2 domain

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

human Nck2 SH2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP9

Plasmid

#175793

Purpose

de novo thick circular tandem repeat proteins with 9 repeats

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRS426-CeLACE-1

Plasmid

#174523

Purpose

Express C.elegans LACE-1 in yeast cells

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

lace-1 (C30F12.2 Nematode)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

MET25

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Lifeact-mEos2-APEX2

Plasmid

#115563

Purpose

Lifeact-mEos2-APEX2 fusion for PALM and CLEM. Parton lab clone IET

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mEos2

Use

Tags

APEX2 and Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2 (GC rich)

Plasmid

#115550

Purpose

Multisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHS

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

APEX2 (GC rich)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-rab10

Plasmid

#109661

Purpose

Multisite gateway vector for 3' tagging with rab10

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab10 (rab10 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab19

Plasmid

#140888

Purpose

Expression of zebrafish GFP-tagged Rab19 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab19 (rab19 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab26 in pCSDEST2

Plasmid

#140884

Purpose

Expression of zebrafish GFP-tagged Rab26 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab26 (rab26 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab40b in pCSDEST2

Plasmid

#140883

Purpose

Expression of zebrafish GFP-tagged Rab40b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab40b (rab40b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13 in pCSDEST2

Plasmid

#140879

Purpose

Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab13 (rab13 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab38a

Plasmid

#140887

Purpose

Expression of zebrafish GFP-tagged Rab38a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38a (rab38a Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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IX001: pBS/Fiber

Plasmid

#131754

Purpose

Plasmid containing Ad5 Fiber gene flanked by homology arms for insertion into the recombinant Ad5 vector IQ013 to generate tropism modified vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Ad5 wild-type Fiber

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

N/A

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pZA17

Plasmid

#158495

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIs8-4b-SaDAH::G226D

Plasmid

#140130

Purpose

Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase G226D variant from Sinomenium acutum

Use

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Mutation

changed Gly226 to Asp226

Promoter

T7

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pME-TCAP

Plasmid

#109549

Purpose

Multisite gateway vector for 5' tagging with telethonin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

telethonin (tcap Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Please see Depositor Comments

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab41

Plasmid

#109767

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab41

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab41 (rab41 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Rab38b

Plasmid

#109757

Purpose

Multisite gateway vector for 3' tagging with Rab38b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38b (rab38b Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Rab38c

Plasmid

#109758

Purpose

Multisite gateway vector for 3' tagging with Rab38c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38c (rab38c Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Rab41

Plasmid

#109759

Purpose

Muscle specific zebrafish expression of mKate2 fused to p3E-Rab41

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab41 (rab41 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-Rab3b

Plasmid

#109760

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab3b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab3b (rab3b Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-Rab19

Plasmid

#109761

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab19

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab19 (rab19 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-Rab27b

Plasmid

#109762

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab27b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab27b (rab27b Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-Rab31

Plasmid

#109763

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab31

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab31 (rab31 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-Rab38b

Plasmid

#109765

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab38b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38b (rab38b Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-Rab38c

Plasmid

#109766

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab38c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38c (rab38c Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab26-DN

Plasmid

#109745

Purpose

Multisite gateway vector for 3' tagging with dominant negative rab26

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab26 (rab26 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

dominant negative

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab26-CA

Plasmid

#109746

Purpose

Multisite gateway vector for 3' tagging with constitutively active rab26

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab26 (rab26 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

constitutively active

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab38a-CA

Plasmid

#109748

Purpose

Multisite gateway vector for 3' tagging with constitutively active rab38a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab38a (rab38a Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

constitutively active

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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