We narrowed to 5,554 results for: donor

1. qTAG-C-mStayGold-Puro-PXN

   Plasmid

   #227317

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mStayGold-Puro-H2BC11

   Plasmid

   #227332

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-moxGFP-Puro-H2BC11

   Plasmid

   #207758

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Epac-S-H81

   Plasmid

   #170339

   Purpose

   GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

   Insert

   GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-miRFP670nano3-Blast-H2BC11

   Plasmid

   #207762

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. qTAG-C-moxGFP-Zeo-H2BC11

   Plasmid

   #207759

   Purpose

   Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

   Plasmid

   #184397

   Purpose

   Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Firefly luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

   Plasmid

   #184398

   Purpose

   Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1-surrogate-reporter

    Plasmid

    #65624

    Purpose

    Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attP
    

    Use

    Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_C2515

    Plasmid

    #91083

    Purpose

    Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    AtU6

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid

    #227306

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_C2518

    Plasmid

    #91087

    Purpose

    Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    TaU6

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Epac-S-H90

    Plasmid

    #170340

    Purpose

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid

    #176458

    Purpose

    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMOD_C2616

    Plasmid

    #91090

    Purpose

    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    At7SL

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD_C2517

    Plasmid

    #91086

    Purpose

    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    TaU3

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_C2520

    Plasmid

    #91089

    Purpose

    Module C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    OsU6

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #196545

    Purpose

    Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:

    Insert

    Surface marker and Citrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC147-pCR8-dCas9VP64

    Plasmid

    #48219

    Purpose

    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP64

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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