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pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

Plasmid

#46676

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-133a-1 (MIR133A1 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pOCC28

Plasmid

#118873

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease and MBP, cleavab…

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP WT

Plasmid

#123080

Purpose

Gateway entry clone encoding human GABARAP wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC291

Plasmid

#118867

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease, mRuby3

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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hLOXL2-S300P

Plasmid

#200061

Purpose

LOXL2 mutant

Depositor

Lakshmi Santhanam

Article

Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

Insert

human lysyl oxidase like 2 (LOXL2 Human)

Expression

Mammalian

Mutation

S300P mutation

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-dCre

Plasmid

#203845

Purpose

Flp-dependent enzymatically inactive Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mRuby3-2A

Mutation

Enzymatically inactive Cre

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-BirA-CPDSalI

Plasmid

#38258

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

birA (birA E. coli)

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pGEX4T1-BirA

Promoter

T7

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204794

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pOCC61

Plasmid

#118877

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette

Tags

mGFP, HIS6, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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