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pAAV-nEF-Coff/Fon-NpHR3.3-EYFP

Plasmid

#137154

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

W179F

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
GIV-F1685A FLAG

Plasmid

#65948

Purpose

Expression GIV/Girdin F1685A mutation in Mammalian cell

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

GIV/Girdin (CCDC88A Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Phenylalanine 1685 to Alanine

Promoter

CMV

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-nEF-Coff/Fon-ChR2-mCherry

Plasmid

#137144

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

H134R

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP

Plasmid

#137148

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing both Cre AND Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/34R]

Plasmid

#220419

Purpose

Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-NALCN [N185/7R]

Plasmid

#177508

Purpose

Mammalian Expression Plasmid of anti-NALCN (Rat). Derived from hybridoma N185/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NALCN (Rattus norvegicus) recombinant mouse monoclonal antibody (Nalcn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-ChR2-mCherry

Plasmid

#137142

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing both Cre AND Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

H134R

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

Plasmid

#137163

Purpose

Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

H134R

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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HC87 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135223

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA-EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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