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We narrowed to 4,130 results for: pCMV mammalian

Showing: 2441 - 2480 of 4130 results

  1. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid
    #208970
    Purpose
    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208970-plasmid-map-sequence-id-415821

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  2. pCMV6-AN-DDK_WT aa 1-317 RAD23A (delta UBA2 domain)

    Plasmid
    #201465
    Purpose
    Expresses a mutant form of human RAD23A that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks C-terminal residues 318-363
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201465-plasmid-map-sequence-id-404913

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  4. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

    Plasmid
    #208972
    Purpose
    Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208972-plasmid-map-sequence-id-415827

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  7. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

    Plasmid
    #208292
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpCas9(D10A)
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208292-plasmid-map-sequence-id-410847

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  8. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid
    #176531
    Purpose
    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.
    Depositor
    Jared Toettcher
    Article
    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13
    Inserts
    KGV transcription factor
    TagBFP
    Use
    Piggybac transposase integration vector
    Tags
    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…
    Expression
    Mammalian
    Available Since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    176531-plasmid-map-sequence-id-375608

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  9. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

    Plasmid
    #208291
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpRY(D10A/…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208291-plasmid-map-sequence-id-410846

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  11. pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid
    #241144
    Purpose
    Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and HaloTag-tdMCP-CAAX to track mature and nascent HA-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-GCN4 scFv
    tdMCP - CAAX
    Tags
    (n2)oxStayGold(c4) E147D and HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    241144-plasmid-map-sequence-id-485248

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  14. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

    Plasmid
    #201546
    Purpose
    Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 188-228
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201546-plasmid-map-sequence-id-395053

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  15. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  16. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

    Plasmid
    #208961
    Purpose
    Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208961-plasmid-map-sequence-id-415791

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  17. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid
    #208943
    Purpose
    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208943-plasmid-map-sequence-id-415668

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  18. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid
    #208944
    Purpose
    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208944-plasmid-map-sequence-id-415672

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  19. pCMV6-AN-DDK_WT RAD23A_aa 111-363 (delta UBL domain)

    Plasmid
    #201460
    Purpose
    Expresses a mutant form of human RAD23A that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks N-terminal residues 1-110
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201460-plasmid-map-sequence-id-404909

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  20. pCMV6-AN-DDK_WT RAD23A_delta aa 161-201 (delta UBA1 domain)

    Plasmid
    #201463
    Purpose
    Expresses a mutant form of human RAD23A that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 161-201
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201463-plasmid-map-sequence-id-395044

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  21. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid
    #192835
    Purpose
    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells
    Depositor
    Karin Reinisch
    Article
    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070
    Insert
    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    Tags
    3xFLAG-PreScission cleavage site and 6xhis
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192835-plasmid-map-sequence-id-379957

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  22. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid
    #208962
    Purpose
    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208962-plasmid-map-sequence-id-415796

    Enlarge

  23. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

    Plasmid
    #208945
    Purpose
    A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208945-plasmid-map-sequence-id-415676

    Enlarge

  31. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid
    #208290
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpCas9(D10…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208290-plasmid-map-sequence-id-410848

    Enlarge

  38. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid
    #101139
    Purpose
    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.
    Depositor
    William Kaelin
    Article
    Lu et al Science. 2014 Jan 17;343(6168):305-9. do
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    101139-plasmid-map-sequence-id-194900

    Enlarge

  39. pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

    Plasmid
    #241142
    Purpose
    Expresses Anti-HA frankenbody-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent HA-tagged proteins and track MS2 and PP7-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-HA frankenbody
    tdMCP
    tdPCP
    Tags
    (n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    241142-plasmid-map-sequence-id-487015

    Enlarge

  40. pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain)

    Plasmid
    #201547
    Purpose
    Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 276-339
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201547-plasmid-map-sequence-id-395051

    Enlarge

Showing: 2441 - 2480 of 4130 results