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We narrowed to 4,275 results for: Pet2

Showing: 3051 - 3100 of 4275 results

  1. pJK500

    Plasmid
    #72245
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and endogenous
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72245-plasmid-map-sequence-id-140434

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  2. pJK345

    Plasmid
    #71559
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 and alanine-368
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71559-plasmid-map-sequence-id-134626

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  3. pJK344

    Plasmid
    #71558
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 (same as deleting alanine-368)
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71558-plasmid-map-sequence-id-134625

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  4. pJK624

    Plasmid
    #72539
    Purpose
    Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72539-plasmid-map-sequence-id-132631

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  5. pJK625

    Plasmid
    #72541
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72541-plasmid-map-sequence-id-132634

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  7. 4QQI

    Plasmid
    #61949
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. 2MJ8

    Plasmid
    #61956
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDYL2 (CDYL2 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. 4LG6

    Plasmid
    #60093
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. L3MBTL1 (3P8H)

    Plasmid
    #28265
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL1 (L3MBTL1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 200-530.
    Promoter
    Available Since
    April 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. CHKB (2IG7)

    Plasmid
    #25516
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CHKB (CHKB Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. ARL10B

    Plasmid
    #25522
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARL10B (ARL8A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. CENTA1 (3LJU)

    Plasmid
    #25340
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CENTA1:K3-F370 (ADAP1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids K3-F370
    Promoter
    Available Since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. CENTA1 (3FEH)

    Plasmid
    #25262
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CENTA1:K3-F370 (ADAP1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids K3-F370
    Promoter
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. WDR5 (2H9O)

    Plasmid
    #25230
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5_01 (WDR5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. XX01CAPN1A-c001

    Plasmid
    #175491
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN1 / CAPNS1 (CAPN1 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175491-plasmid-map-sequence-id-353165

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  19. XX01CAPN2A-c001

    Plasmid
    #175492
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN2 / CAPNS1 (CAPN2 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175492-plasmid-map-sequence-id-353163

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  20. pET_RTX(exo-)-6xHis

    Plasmid
    #145028
    Purpose
    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX(exo-)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    N210D
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    145028-plasmid-map-sequence-id-292638

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  22. WT FL USP7

    Plasmid
    #227214
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227214-plasmid-map-sequence-id-452331

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  27. CAPN1

    Plasmid
    #25607
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CAPN1 (CAPN1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 29-360
    Promoter
    Available Since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. EHMT2

    Plasmid
    #25503
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EHMT2 (EHMT2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. AK3L1

    Plasmid
    #25541
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AK3L1 (AK4 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. FKBP8

    Plasmid
    #25609
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FKBP8 (FKBP8 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    FKBP-type PPIase Domain, residues 90-205
    Promoter
    Available Since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25609-plasmid-map-sequence-id-454823

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  34. GCN5L2

    Plasmid
    #25482
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GCN5L2 (KAT2A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. EIF5A

    Plasmid
    #25260
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    eIF5A:S15-A151 (EIF5A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S15-A151
    Promoter
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. 6CC8 (MBD3)

    Plasmid
    #119959
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD3 (MBD3 Human)
    Use
    Tags
    GSTtag, 6xHis and Thrombin
    Expression
    Bacterial
    Mutation
    MBD domain (contains residues 1-71)
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119959-plasmid-map-sequence-id-232658

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  46. pJYP1

    Plasmid
    #202406
    Purpose
    Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)
    Depositor
    Remy Loris
    Article
    Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    fdeA
    Available Since
    Aug. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202406-plasmid-map-sequence-id-403395

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  47. SUMO-KTag

    Plasmid
    #51723
    Purpose
    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SUMO-KTag
    Use
    Tags
    His6 Tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  48. GABARAPL1

    Plasmid
    #25215
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh57.003.111 (GABARAPL1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. 5WCI (MYST1)

    Plasmid
    #101269
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST1 (KAT8 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101269-plasmid-map-sequence-id-193502

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  50. 5TTW (EED)

    Plasmid
    #101254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EED (EED Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101254-plasmid-map-sequence-id-197525

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Showing: 3051 - 3100 of 4275 results