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pFGC5941-PacI-ath-STTM866-3p

Plasmid

#84187

Purpose

target miRNA866-3p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM866-3p

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA-H1-sgRNA-mTZAP

Plasmid

#87187

Purpose

guideRNA targeting exon2 of mouse TZAP

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (Zbtb48 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLN432 (FuGW-G14p-mKate2)

Plasmid

#105187

Purpose

Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G14p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pGTMV

Plasmid

#118755

Purpose

Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.

Depositor

Jose-Antonio Daros

Article

Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

Insert

Tobacco mosaic virus

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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LB601: pMVP/PB/eGFP-DEST

Plasmid

#121870

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSP2-96-merTetO-EFS-BLaR

Plasmid

#118713

Purpose

Contains an optimized 96-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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VPS13A^Halo

Plasmid

#118759

Purpose

VPS13A internally tagged with Halo at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Use

Tags

Halo tag inserted at position 1372

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET-15b-TEV-C9R

Plasmid

#118754

Purpose

TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

Depositor

Yutaka Kuroda

Article

Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

Insert

Tobacco Etch Virus protease (NEWENTRY Synthetic)

Use

Tags

C9R and His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBOB-VE-cadherin-GFP

Plasmid

#118732

Purpose

To express VE-cadherin tagged with GFP on C-terminus

Depositor

John Cooper

Article

Kim et al Mol Biol Cell. 2018 Jul 15;29(13):1693-1

Insert

Cadherin 5 (CD144) (CDH5 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

I503T (Common SNP-see comments)

Promoter

CMV

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR5'_ubi

Plasmid

#27320

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

zebrafish ubiquitin promoter (ubb Zebrafish)

Use

Multisite gateway 5' entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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Human SERCA2a (pcDNA3.1+)

Plasmid

#75187

Purpose

Mammalian expression of ATP2A2

Depositor

David MacLennan, Jonathan Lytton

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCM184-ParB2::eGFP

Plasmid

#87252

Purpose

Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB2

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

pBAD/tetO

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCM189-ParB1::mCherry

Plasmid

#87253

Purpose

Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB1

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pBAD/tetO

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMTMVi-M

Plasmid

#118757

Purpose

Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.

Depositor

Jose-Antonio Daros

Article

Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

Insert

Tobacco mosaic virus cDNA fragment with a cloning polylinker

Use

Intermediate to build tobacco mosaic virus recomb…

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1147 GFP-TDP43

Plasmid

#118797

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pTight-9-124

Plasmid

#31874

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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