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Plasmid#192833PurposeExpress codon-optimized human MIGA2 fragment 170-593 in Expi293 cellsDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags3xFLAG-PreScission cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190078Purposeexpress Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (TMPRSS2 Rhinolophus ferrumequinum)
UseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127_P
Plasmid#147220PurposeBacterial Expression of DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127DepositorInsertDmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin
Plasmid#182305PurposeE. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.DepositorInsertpH-Countdown insertion into GECO 1.2 calcium sensing domains
UseTagsA modified TorA periplasmic targeting sequence pl…ExpressionBacterialMutationSpontaneous mutation of calmodulin's penulti…Promotert7Available sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508del231-254_GST-HsRCK_70-472_D
Plasmid#146108PurposeBacterial Expression of HsEDC3_192-508del231-254-HsRck_70-472DepositorInsertHsEDC3_192-508del231-254-HsRck_70-472 (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-C4A,C5A,C7A-GFP
Plasmid#166838PurposeExpresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationC4A, C5A, C7APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]
Plasmid#106462PurposeExpresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W(N163,242Q)
UseTagsPAExpressionBacterial and YeastMutationchanged Asparagines at positions 163 and 242 to G…PromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pED55 pRS306 pRPS2-SteND-NLS-mCherry
Plasmid#68277PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutationDepositorInsertSte7ND NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry
Plasmid#68275PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLSDepositorInsertSte7DS NLS(4A) mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB1 Cterminal tail
Plasmid#60400PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail
Plasmid#60397PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationC127S; TUB2 amino acids 429 - end, replaced with …PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB7 Cterminal tail
Plasmid#60399PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB8 Cterminal tail
Plasmid#60401PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail
Plasmid#60402PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-PR-LBD
Plasmid#89117Purposebacterial expression of human progesterone receptor ligand binding domain amino acid residues 637-933 with GST tag in pGEX-3X-1DepositorInserthuman progesterone receptor ligand binding domain (PGR Human)
UseTagsGSTExpressionBacterialMutationcontains Ligand Binding Domain (637-933)PromotertacAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR-spondin 1
Plasmid#128844Purposebacterial expression of pR-spondin 1DepositorInsertR-spondin 1 (RSPO1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/GFP-PR
Plasmid#20253DepositorInsertHIV-1 protease
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.2/pr-Membrane
Plasmid#120817PurposeEctopic expression of ZIKV proteinDepositorInsertpr-Membrane ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPath-pR-integrase
Plasmid#200000PurposeEntry vector for biosynthetic pathway. pR-phiC31 integraseDepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only