We narrowed to 1,322 results for: pET28a

1. pET28-Mff(1-61)-PP-GST

   Plasmid

   #73042

   Purpose

   Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Mff(1-61) (Mff Mouse)
   

   Use

   Tags

   GST-tag and PreScission protease site

   Expression

   Bacterial

   Mutation

   Truncation containing amino acids 1-61

   Promoter

   T7

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_human-pro-IL-18_DeltaN24

   Plasmid

   #214327

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Deletion of first 24 amino acids

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-HISx6-SIMx6

   Plasmid

   #116938

   Purpose

   Bacterial expression vector for 6 sim

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   6 sim
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-HISx6-SUMOx10

   Plasmid

   #116937

   Purpose

   Bacterial expression vector for 10 sumo

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   10 SUMO
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. H6SUMO-IntC-P53(304-393)

   Plasmid

   #200316

   Purpose

   bacterial expression of P53 TET-CTD for segmental labeling

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (304-393) (TP53 Human)
   

   Use

   Tags

   His6-SUMO and designed intein C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_Human_SAT1_Cterm_His

   Plasmid

   #220487

   Purpose

   heterologous expression of SSAT1 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   SSAT1 (SAT1 Human)
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_Human_SAT1_Nterm_His

   Plasmid

   #220482

   Purpose

   heterologous expression of SSAT1 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   SSAT1 (SAT1 Human)
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Human_TFAM_dHMGA_pET28

   Plasmid

   #167329

   Purpose

   Expresses TFAM dHMGA mutant in bacterial cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Truncation mutant containing linker, HMGB, and C-…

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YFP-FKBPx5-His

   Plasmid

   #103785

   Purpose

   expresses His-tagged iPOLYMER component in E. coli for protein purification

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   YFP-FKBPx5-His
   

   Use

   Tags

   EYFP, His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HOXB2 (human) HIS-tag pET

    Plasmid

    #8522

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB2 (HOXB2 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at AA #2; makes protein by Western

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pET28-p53 (1-73) (72P)

    Plasmid

    #62083

    Purpose

    Expression of human p53 (1-73) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72P

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

12. HOXB1 (human) HIS-tag pET

    Plasmid

    #8520

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB1 (HOXB1 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    PCR from cDNA clone

    Promoter

    Available Since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

13. p28atDarpp

    Plasmid

    #108318

    Purpose

    Expresses tDarpp in E.coli

    Depositor

    Jamil Momand

    Article

    Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

    Insert

    tDarpp (PPP1R1B Human)
    

    Use

    Tags

    6 Histadine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

15. 4E2H.210_LUMABS

    Plasmid

    #155209

    Purpose

    Expresses 4E2H.210_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210_LUMABS
    

    Use

    Tags

    6x His Tag

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. HOXD4 (murine) HIS-tag pET

    Plasmid

    #8548

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western and by in vitro transcri…

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pET-28a-His6-SUMO-RABIF

    Plasmid

    #135556

    Purpose

    Express human RABIF in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    RABIF (RABIF Human)
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK597

    Plasmid

    #71121

    Purpose

    Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. p47 PX(1-121)/p40phox(136-339)

    Plasmid

    #19018

    Depositor

    Michael Yaffe

    Article

    Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

    Insert

    p40phox/p47phox (NCF4, NCF1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    insert containing amino acids 1-121 of p47phox fu…

    Promoter

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

20. hSTX4(1-271,272C)

    Plasmid

    #206031

    Purpose

    Bacterial expression of human Syntaxin 4 without transmembrane helix and a C-terminal cysteine

    Depositor

    Geert van den Bogaart

    Article

    Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

    Insert

    Syntaxin-4 (STX4 Human)
    

    Use

    Tags

    his-tag (removable with thrombin)

    Expression

    Bacterial

    Mutation

    deleted amino acids 272-297 (encoding the transme…

    Promoter

    T7

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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