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pAtCas9-D10A_1

Plasmid

#91159

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTC366

Plasmid

#91215

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0808

Plasmid

#91024

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0408

Plasmid

#91010

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0708

Plasmid

#91021

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1_spikeIn-2

Plasmid

#86388

Purpose

Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter

Use

Stap-seq spike in vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HOMEZ_1

Plasmid

#86352

Purpose

Encodes gRNA for 3' target of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HOMEZ (HOMEZ Human)

Use

CRISPR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF652_2

Plasmid

#86317

Purpose

Encodes gRNA for 3' target of human ZNF652

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF652 (ZNF652 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HOMEZ

Plasmid

#86289

Purpose

Donor vector for 3' FLAG tag of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HOMEZ homology arms (HOMEZ Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HOMEZ_2

Plasmid

#86353

Purpose

Encodes gRNA for 3' target of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HOMEZ (HOMEZ Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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