We narrowed to 6,121 results for: pcDNA 3.1

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(133) pcDNA3.1-Nup62-Flag-EPEA

Plasmid

#160744

Purpose

Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

Nup62-Flag-EPEA

Tags

EPEA and Flag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Linear Tau 0N4R 3X Flag tag

Plasmid

#194171

Purpose

Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-Associated Protein Tau 0N4R

Tags

3X Flag

Expression

Mammalian

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

Plasmid

#182651

Purpose

human CD81-TEV cleavage site-FKBP-SmBiT tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)

Tags

SmBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

Plasmid

#69908

Purpose

Expresses a GFP circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1, GFP (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

Plasmid

#225587

Purpose

Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

T21R-vhhGFP4-T2A-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG

Plasmid

#169991

Purpose

Mammalian purification construct for CARD8

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG

Plasmid

#225240

Purpose

Mammalian expression of iPASnFR-L in mitochondria

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225239

Purpose

Mammalian expression of iPASnFR-H in mitochondria

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

Plasmid

#194680

Purpose

CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Hpep1-betaArrestin2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tension Sensor

Plasmid

#182717

Purpose

expresses DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al iScience. 2025 Jul 9;28(8):113081. doi:

Insert

desmoglein-3 (Dsg3 Mouse)

Expression

Mammalian

Mutation

TSmod inserted between G726 and G727

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS Exon Vector

Plasmid

#69893

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pplss-mRFP-KDEL-pcDNA3.1/Hygro

Plasmid

#242808

Purpose

ER marker

Depositor

Christoph Kaether

Insert

mRFP with ER targeting signal sequence and C-terminal KDEL-ER retention signal

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA 3.1 Flag-rat-PTP1B

Plasmid

#35988

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG

Plasmid

#225246

Purpose

Mammalian expression of iPASnFR-L in the nucleus

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

Plasmid

#171437

Purpose

Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

c-Fos-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225245

Purpose

Mammalian expression of iPASnFR-H in the nucleus

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

Plasmid

#171438

Purpose

Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-c-Jun

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEEF1D-FLAG (pcDNA 3.1+) LG6-1

Plasmid

#37365

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 (+) -myc-OGA(GS-544)C181

Plasmid

#193996

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181

Tags

myc

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) anti-Her2 (FRP5) scFv-Fc

Plasmid

#183249

Purpose

Soluble expression of anti-human Her2 scFvFc (clone FRP5). Human IgG1 Fc.

Depositor

Alexander McLellan

Insert

Anti-Her2 scFvFc (FRP5)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag YFP hNKCC1 (NT17)

Plasmid

#49085

Purpose

Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

Depositor

Biff Forbush

Article

Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

Insert

human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)

Tags

3x Flag and mVenus

Expression

Mammalian

Mutation

262 silent mutations from native hNKCC1

Promoter

CMV

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

Plasmid

#158953

Purpose

Express human RAD51(T309A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed threonine 309 to alanine

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-NLRP1 codon-optimized

Plasmid

#166833

Purpose

Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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XE248 pCDNA3.1+zeo-VSV-KLHL12

Plasmid

#16761

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

pCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)

Tags

VSV

Expression

Mammalian

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

Plasmid

#171436

Purpose

Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-Lifeact

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KozATG-dL5-2XG4S-mCer3

Plasmid

#73207

Purpose

Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

Insert

KozATG-dL5-2XG4S-mCer3

Tags

The FAP and mCerulean3 are fused with 2 copies of…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-SIV-MAC251-vpx

Plasmid

#115842

Purpose

Encodes codon optimized FLAG tagged SIVMAC251-Vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251-Vpx

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG

Plasmid

#166803

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

Plasmid

#166794

Purpose

Mammalian cell expression vector for Flag-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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