Addgene

We narrowed to 84,520 results for: MAL

Showing: 2481 - 2520 of 84520 results

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pRK-flag-USP19

Plasmid

#78597

Purpose

Express flag-USP19 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3-mRFP

Plasmid

#184902

Purpose

Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7, monomeric EGFP, and monomeric RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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mCitrine-USP19

Plasmid

#78593

Purpose

Express mCitrine-USP19 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 (USP19 Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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epB_UbC_CymRV5-nls-Halox2_DEx4

Plasmid

#119907

Purpose

ePiggyBac mammalian expression plasmid for CymR-Halo fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-Halox2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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c-Rel cFlag pcDNA3

Plasmid

#20013

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

c-Rel (Rel Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLOVE-GFP

Plasmid

#15949

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

Insert

Green Fluorescent Protein

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Promoter

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-NanoLuc

Plasmid

#188313

Purpose

Expression of NanoLuc via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

NanoLuc

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pmNeonGreenHO-mito

Plasmid

#127913

Purpose

a mammalian expression vector for mitochondria-targeting humanized mNeonGreen

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen with mitocondrial targetting signal

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-AKAP-mtagBFP2-UBC6

Plasmid

#105007

Purpose

Mammalian Expression of mtagBFP2

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

mtagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:WTIP

Plasmid

#108231

Purpose

Lentiviral vector for Tet-inducible WTIP expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

WTIP (WTIP Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Tet/ON Minimal CMV promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pIM23

Plasmid

#74643

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

MET25

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 WT in pcDNA3.1

Plasmid

#118213

Purpose

Expresses HA-UBE2E1 in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Tags

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_124

Plasmid

#133461

Purpose

EF1a promoter expresses SpyoCas9 and P2A provides EGFP

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

SpyoCas9

Use

CRISPR and Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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p458 VQR

Plasmid

#101727

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Use

Tags

Expression

Mammalian

Mutation

VQR

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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epB_CAG_TetRFlag-nls-tdTom_DEx4

Plasmid

#119909

Purpose

ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

TetRFlag-nls-tdTom

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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tTA_BFP

Plasmid

#58854

Purpose

Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

tTA transcription factor fused to enhanced blue fluorescent protein

Use

Tags

BFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-L1T1L-NLuc

Plasmid

#128942

Purpose

Expresses L1-NLuc gene

Depositor

Takeshi Kobayashi

Article

Kanai et al J Virol. 2019 May 8. pii: JVI.00401-19.

Insert

L1 minor core protein

Use

Tags

Nanoluc

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-L26-Flag

Plasmid

#19972

Depositor

Moshe Oren

Article

Ofir-Rosenfeld et al Mol Cell. 2008 Oct 24. 32(2):180-9.

Insert

Rpl26 (RPL26 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only
mCitrine-Rab9a

Plasmid

#78596

Purpose

Express mCitrine-Rab9a in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

Rab9a (RAB9A Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pL7Ae-myc-T2A-tagRFP

Plasmid

#140263

Purpose

Expresses L7Ae in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

L7Ae

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-RNF126

Plasmid

#138643

Purpose

Bacterial expression of GST-RNF126 fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS2CP-myc-T2A-tagRFP

Plasmid

#140259

Purpose

Expresses WT MS2CP in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

Coat protein of bacteriphage MS2

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pU1A_full-myc-T2A-tagRFP

Plasmid

#140264

Purpose

Expresses U1A in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

U1A

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIR_PKAbiosensor

Plasmid

#127439

Purpose

Mammalian expression of the NIR biosensor for detecting PKA activity

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

NIR PKA biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIR_JNKbiosensor

Plasmid

#127440

Purpose

Mammalian expression of the NIR biosensor for detecting JNK activity

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

NIR JNK biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MCP-VPR-2AmScarlet

Plasmid

#121964

Purpose

Mammalian expression, Transcription regulation module

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPP7CP-myc-T2A-tagRFP

Plasmid

#140262

Purpose

Expresses PP7CP in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

Coat protein of bacteriphage PP7

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-TMF1i2

Plasmid

#59625

Purpose

Expression of shRNA against human TMF1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

TMF1 (TMF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-TMF1i1

Plasmid

#59624

Purpose

Expression of shRNA against human TMF1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

TMF1 (TMF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pUdOm1.0

Plasmid

#210298

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Myo 1b-myc

Plasmid

#135064

Purpose

Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

Insert

Myo 1b with 5' UTR (Myo1b Rat)

Use

Tags

Linker and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2481 - 2520 of 84520 results

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