We narrowed to 3,154 results for: FRI

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pCAG-HA-NLGN3 R451C

Plasmid

#59319

Purpose

Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN3 R451C (NLGN3 Human)

Tags

HA

Expression

Mammalian

Mutation

Arginine 451 to Cysteine (R451C mutation)

Promoter

CAG

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAD/CMV/V5-DEST-Cdc42-2G

Plasmid

#68812

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Adenoviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLJC2-MTHFD1-3xFLAG

Plasmid

#217429

Purpose

Lentiviral overexpression of human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

MTHFD1 (MTHFD1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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plko-shmfn2-mito-GFP-ER tethering

Plasmid

#160509

Purpose

Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mito-GFP-ER tethering

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBS4S

Plasmid

#55170

Purpose

Empty vector, integration at thrC, ampr, specr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hSynapsin1-axon-GCaMP6s

Plasmid

#111262

Purpose

For enriched expression of GCaMP6s in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6s

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLV.CMV.flex.hM3Dq.Cherry

Plasmid

#170360

Purpose

Cre-inducible lentiviral vector expressing hM3Dq

Depositor

Johan Jakobsson

Article

Klawonn et al Immunity. 2021 Feb 9;54(2):225-234.e6. d

Insert

flex.hM3Dq.Cherry

Use

Lentiviral

Promoter

CMV

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVf-EnhCB-lacZnls miR-122 3x

Plasmid

#35644

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

AAV

Promoter

CB

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Ago1

Plasmid

#21534

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pGI3EM11

Plasmid

#135483

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-GFP ADH1t

Tags

GFP

Expression

Bacterial and Yeast

Promoter

SpunH2Bpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM18

Plasmid

#135484

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-tdTomato ADH1t

Tags

tdTomato

Expression

Bacterial and Yeast

Promoter

SpunH2Bpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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Barcode backbone

Plasmid

#158665

Purpose

This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Type

Empty backbone

Use

Lentiviral

Tags

hcRED

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTomo-Ras

Plasmid

#26292

Depositor

Inder Verma

Article

Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

Insert

HRAS-V12 (HRAS Human)

Use

Lentiviral

Tags

Flag

Mutation

G12V

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E IRES GFP

Plasmid

#18761

Depositor

Scott Lowe

Article

Wendel et al Nature. 2004 Mar 18. 428(6980):332-7.

Insert

eIF4E (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
sIL6R PLAK1

Plasmid

#161519

Purpose

Expresses soluble IL6 receptor in mammalian cells.

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

soluble IL-6 receptor

Use

Lentiviral

Tags

3X Flag

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6f

Plasmid

#112000

Purpose

For enriched expression of GCaMP6f in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6f

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGI3EM22C

Plasmid

#135488

Purpose

Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr LifeAct-tdTomato

Tags

LifeAct

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-His-MinD_MinE

Plasmid

#133621

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

Depositor

Petra Schwille

Article

Loose et al Science. 2008 May 9;320(5877):789-92. do

Insert

His-MinD_MinE (minD, minE E. coli)

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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