We narrowed to 11,220 results for: NSI

1. p15_attB(bxb)_lox

   Plasmid

   #183764

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TfR-pHoran4

   Plasmid

   #61547

   Purpose

   Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

   Depositor

   David Perrais

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   Transferrin receptor (TFRC Human)
   

   Use

   Tags

   pHoran4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

   Plasmid

   #229791

   Purpose

   Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRetroX-Tight-MCS_PGK-GpNLuc

   Plasmid

   #70185

   Purpose

   Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetroX-Tight-MCS_PGK-OgNLuc

   Plasmid

   #70186

   Purpose

   Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXJ-His-VTCN1

   Plasmid

   #232664

   Purpose

   transient expresses VTCN1 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   VTCN1 (VTCN1 Human)
   

   Use

   Tags

   His and His (retained after signal peptide cleava…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. mbYFPQS

   Plasmid

   #80742

   Purpose

   membrane-targeted biosensor for monitoring changes in intracellular chloride

   Depositor

   Susan Ingram, Susan Amara

   Article

   Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

   Insert

   YFPQS
   

   Use

   Tags

   palmitoylation signal sequence of neuromodulin

   Expression

   Mammalian

   Mutation

   H148Q (CAC to CAG) and V163S (GTG to AGC).

   Promoter

   CMV

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

8. Yellow Cameleon-Nano15/pBig

   Plasmid

   #51962

   Purpose

   Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano15
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBR_attB(C31)_FRT

   Plasmid

   #183761

   Purpose

   phiC31-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Bxb1-GT_CAG_3xTurboGFP-NLS

    Plasmid

    #229777

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    3x TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-HBB-nLuc

    Plasmid

    #175432

    Purpose

    Used to generate IVT RNA for translation extracts

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    HBB-nLuc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Emi LC

    Plasmid

    #113666

    Purpose

    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.

    Depositor

    Robert Peters

    Article

    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

    Insert

    Emicizumab light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET19b-EcMscL-TEV-mEGFP

    Plasmid

    #165097

    Purpose

    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

    Depositor

    Neha Kamat

    Article

    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

    Insert

    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    

    Use

    Synthetic Biology

    Tags

    monomeric Enhanced Green Fluorescent Protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bxb1-GA_CAG-TurboGFP

    Plasmid

    #229781

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Yellow Cameleon-Nano140/pcDNA3

    Plasmid

    #51966

    Purpose

    mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano140
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GA_CAG-ITPKA-3x-mScarlet-I

    Plasmid

    #229782

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    3x mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    ITPKA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWaldo-GFPe_PepTSo

    Plasmid

    #58334

    Purpose

    Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    So Iwata, Simon Newstead

    Article

    Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

    Insert

    PepTso
    

    Use

    Tags

    GFP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pET22b_EcSSB_1

    Plasmid

    #78203

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

    Insert

    ssb (G26C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G26C (please see depositor comment below)

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Yellow Cameleon-Nano50/pcDNA3

    Plasmid

    #51964

    Purpose

    mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUWR-CadTS-C

    Plasmid

    #58326

    Purpose

    Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    E-cadherin tension sensor control
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    poly-ubiquitin

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only