Addgene

Showing: 1241 - 1260 of 2504 results

Results per page:

Sort results by:

pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

Plasmid

#203840

Purpose

Kir2.1 expression for silencing neurons and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Expression

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

Plasmid

#225242

Purpose

Mammalian expression of iPASnFR-L in the cytosol

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG

Plasmid

#225246

Purpose

Mammalian expression of iPASnFR-L in the nucleus

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

Plasmid

#170712

Purpose

dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Inserts

mNeonGreen
mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TNNT2 and aMHC

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Flag-ER-Hoxb8-IRES-GFP

Plasmid

#222292

Purpose

Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

Mark Kamps, David Sykes

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Promoter

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

H231L

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV 4xSTAR-mScarletI-NLS-blast

Plasmid

#136260

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mScarletI-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)

Plasmid

#191451

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

N-WASP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225241

Purpose

Mammalian expression of iPASnFR-H in the cytosol

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_hSyn-DIO-PdCO-mScarlet-WPRE

Plasmid

#198511

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TurboID-MCS MSCV-IRES-GFP

Plasmid

#207958

Purpose

Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

Depositor

Timothy Ley

Article

Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

Insert

TurboID

Use

Retroviral; Turboid biotin proximity ligase

Tags

TurboID

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVTR(T7A)-PuroR-T2A-mScarlet

Plasmid

#172301

Purpose

In vitro transcription of PuroR-T2A-mScarlet synthetic construct

Depositor

Patrik Verstreken

Insert

PuroR-T2A-mScarlet

Use

Other

Tags

Expression

Mutation

Promoter

T7 promoter A-initiating

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

Plasmid

#225244

Purpose

Mammalian expression of iPASnFR-L fused to Rab5

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Flt3-ITD-Y591F/Y919F

Plasmid

#74499

Purpose

Retroviral expression of FLT3 ITD-Y591F/Y919F

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

unknown

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-Spe4-RfA-mScarlet-I-stop

Plasmid

#186393

Purpose

Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-MSCV-4xS1m-GFP-T2A-PU

Plasmid

#138581

Purpose

For in vivo tagging

Depositor

Yin-Yuan Mo

Article

Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

Type

Empty backbone

Use

Lentiviral

Tags

S1m tag

Expression

Mutation

Promoter

MSCV

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225239

Purpose

Mammalian expression of iPASnFR-H in mitochondria

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hU6-pegRNA-EFS-mScarlet Template

Plasmid

#196037

Purpose

The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6 and EFS

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge