We narrowed to 1,322 results for: pET28a

1. His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)

   Plasmid

   #243822

   Purpose

   Munc13-1 protein purification

   Depositor

   Jose Rizo-Rey

   Article

   Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

   Insert

   Munc13-1 (Unc13a Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   delta1408-1452 delta1533-1551 R1598E F1658E

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a(+)-6xHis-TEV-ORF9c

   Plasmid

   #201023

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   open reading frame 9c
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human_TFAM_HMGA_linker_pET28

   Plasmid

   #167327

   Purpose

   Expresses TFAM HMGA+linker mutant in bacterial cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Truncation mutant containing HMGA and linker doma…

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human_TFAM_HMGB_ctail_pET28

   Plasmid

   #167330

   Purpose

   Expresses TFAM HMGB+C-tatil mutant in bacterial cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Truncation mutant containing HMGB and C-tail doma…

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human_TFAM_dC_pET28

   Plasmid

   #167328

   Purpose

   Expresses TFAM dC-tail mutant in bacterial cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Truncation mutant containing HMGA, linker and HMG…

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4E1H.95_LUMABS

   Plasmid

   #155208

   Purpose

   Expresses 4E1H.95_LUMABS sensor in E.coli

   Depositor

   Bruno Correia

   Article

   Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

   Insert

   4E1H.95_LUMABS
   

   Use

   Tags

   6x His Tag

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-MBP-super TEV protease

   Plasmid

   #171782

   Purpose

   Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   MBP-sTEV
   

   Use

   Tags

   Arg6 tag and Internal His6 tag

   Expression

   Bacterial

   Mutation

   No internal TEV cleavage site between the MBP and…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6-pAG-RTase

   Plasmid

   #214932

   Purpose

   Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

   Depositor

   Chuan He

   Article

   Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

   Insert

   fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

   Promoter

   T7 promoter

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PARP1 FL

   Plasmid

   #169815

   Purpose

   Expresses codon optimised full length His tagged PARP1 in bacterial cells

   Depositor

   David Neuhaus

   Article

   Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

   Insert

   Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
   

   Use

   Tags

   MKHHHHHHMKQ

   Expression

   Bacterial

   Mutation

   Valine 762 mutated to an alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28:LanIII

    Plasmid

    #225075

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIII)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28:LanIII-43

    Plasmid

    #225077

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIII-43)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28:LanII

    Plasmid

    #225066

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. proISG15 (2-165)

    Plasmid

    #110762

    Purpose

    Bacterial expression of proISG15

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRD421

    Plasmid

    #163112

    Purpose

    Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
    

    Use

    Tags

    His-tag with TEV-cleavable linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Brg1 Del4 pET28C

    Plasmid

    #122118

    Purpose

    Expression Brg1 amino acids 1446-1647 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHelA12

    Plasmid

    #176493

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

    Depositor

    Jian-Dong Huang, Xiaodong Cheng

    Article

    Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

    Insert

    SARS-CoV NSP13 (ORF1ab SARS-CoV)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    V570I

    Promoter

    T7

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. HOXB3 (human) HIS-tag pET

    Plasmid

    #8524

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. C-His-GvpC-Calpain

    Plasmid

    #153295

    Purpose

    Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Calpain-sensitive Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) Contains an extra glycine after the methionine…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. N-His-GvpC-ssrA

    Plasmid

    #153297

    Purpose

    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    

    Use

    Tags

    His-tag and ssrA tag

    Expression

    Bacterial

    Mutation

    1) N-terminal 6xHis tag flanked by 1x glycine res…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. HOXB4 (human) HIS-tag pET new RBS

    Plasmid

    #8525

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains improved Ribosomal binding site; makes …

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only