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Plasmid#73879PurposeChimaera 9DepositorInsertCHI-9
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415 CHI-7
Plasmid#73869PurposeChimaera 7 (COPD-Ret2)DepositorInsertChimaera 7
UseTagsExpressionYeastMutationPromoterMet25Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415 CHI-1
Plasmid#73863PurposeChimaera 1 (Ret2-COPD)DepositorInsertChimaera 1
UseTagsExpressionYeastMutationPromoterMet25Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG272-eya
Plasmid#99085PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea eyes absent (eya)DepositorInserteyes absent (eya)
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-LGK
Plasmid#81165PurposepETconNK with levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
UseTagsc-mycExpressionBacterial and YeastMutationPromoterGAL1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TU#1412_tiam-1_cDNA
Plasmid#82364Purposeexpress tiam-1 cDNADepositorInserttiam-1 cDNA (tiam-1 Nematode)
UseTagsExpressionWormMutationPromoternoneAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
TU#1449_unc-73e_cDNA
Plasmid#82363Purposeexpress unc-73e cDNADepositorInsertunc-73 e isoform (unc-73 Nematode)
UseTagsExpressionWormMutationPromoternoneAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT7-1 (E1083Q)-intein-CBD
Plasmid#73303PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationE1083QPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD
Plasmid#73299PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationS1079N-E1083QPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD437
Plasmid#63758Purpose7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)DepositorInserteae
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD434
Plasmid#63757PurposeEPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.DepositorInserteae probe
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET:CACT:GFP
Plasmid#60737PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-GFP-Patronin CC 534–868
Plasmid#59054Purposefor E. coli expression of Patronin CC truncationDepositorInsertPatronin (Patronin Fly)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1148
Plasmid#53006PurposepCon:OxyS, pLlacO-1:sodB::gfpDepositorInsertsOxyS sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1070
Plasmid#53002PurposepCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsDsrA sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1057
Plasmid#53001PurposepCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsRhyB sRNA
rpoS
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontainsn −150 to +30 bp relative to start codonPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1757
Plasmid#53037PurposePconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterPconNoHindM2 and pConNoHindAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Slc25a15 mod
Plasmid#48161Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from elementDepositorInsert5" UTR of Slc25a15
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Slc25a15
Plasmid#48160Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Slc25a15
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only