161,255 results

1. 9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

   Plasmid

   #209293

   Purpose

   mitoBE expression vector, target mtND4 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-Sox2

   Plasmid

   #13367

   Purpose

   Retroviral expression of mouse Sox2

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   SRY-box containing gene 2 (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGfa2(ABDx3)-nLac

   Plasmid

   #53133

   Purpose

   expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells

   Depositor

   Michael Brenner

   Article

   de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.

   Insert

   Gfa2 (GFAP Human)
   

   Use

   Mouse Targeting

   Tags

   LacZ, NLS, and mP1

   Expression

   Mammalian

   Mutation

   -2163 to +47, with 3 copies of 3 copies of the gf…

   Promoter

   human Gfa2

   Available since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

   Plasmid

   #207953

   Purpose

   nuclease inactive control for TRF1FokI WT

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI (D450A)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

   Plasmid

   #179459

   Purpose

   Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Has Service

   AAV1

   Insert

   JEDI-2P-Kv
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

   Plasmid

   #209294

   Purpose

   mitoBE expression vector, target mtRNR2 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   SOD2-3xHA-TALE(RNR2-Left)-MutH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHO4d-Cas9

   Plasmid

   #67881

   Purpose

   Cas9nlsHis6 Bacterial Expression construct

   Depositor

   Michael Nonet

   Article

   Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and T7 epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-Sestrin2

   Plasmid

   #72593

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

9. CMV-ER-LAR-GECO1

   Plasmid

   #61244

   Purpose

   Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

   Insert

   LAR-GECO1
   

   Use

   Tags

   ER-retention sequence: KDEL and ER-targeting sequ…

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1: V51W/I113V/N35…

   Promoter

   CMV

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV SYN PSAM4 GlyR IRES EGFP

    Plasmid

    #119742

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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