172,049 results

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pEGFP-BUBR1

Plasmid

#234704

Purpose

expression of fusion protein

Depositor

Lienhard Schmitz

Article

Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

Insert

BUBR1 (BUB1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

Plasmid

#235608

Purpose

Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms

Use

CRISPR

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_L31_CRISPR_downstream_sgRNA

Plasmid

#235610

Purpose

Encodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus downstream sgRNA

Use

CRISPR

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_L31_CRISPR_upstream_sgRNA

Plasmid

#235609

Purpose

Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus upstream sgRNA

Use

CRISPR

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2

Plasmid

#229738

Purpose

Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1318 - pAAV-AiE2013m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230527

Purpose

AiE2013m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12582 - pAAV-AiE0486m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2582)

Plasmid

#229894

Purpose

AiE0486m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MST1

Plasmid

#229417

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

STK4 (MST1) (MST1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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9_T7-mScarlet3-LactC2

Plasmid

#225936

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

mScarlet3-LactC2

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12423 - pAAV-AiE0461m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2423)

Plasmid

#223992

Purpose

AiE0461m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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