173,219 results

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pLKO-puro FLAG SREBP1

Plasmid

#32017

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

SREBP1 (Srebf1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

V225M

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pUCKanR-Mu-BsaI

Plasmid

#79769

Purpose

Transposon-propagation plasmid for Mu-BsaI modified transposon

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Mu-BsaI

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human 7SK

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pC016 - LwCas13a guide expression backbone with U6 promoter

Plasmid

#91906

Purpose

Backbone for cloning LwCas13a guides under U6 promoter

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Has Service

Cloning Grade DNA

Insert

LwCas13a Guide site

Expression

Mammalian

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-NanoLuc

Plasmid

#188247

Purpose

Template plasmid to transcribe mRNA encoding NanoLuc

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Expression

Mammalian

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-FLT3

Plasmid

#23895

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT3 (FLT3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry-IRES-Cre

Plasmid

#55632

Purpose

Expresses Cre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Inserts

mCherry
Cre

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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