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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,418 results for: construct

Showing: 631 - 640 of 8418 results

  1. pJK632

    Plasmid
    #72465
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to valine, tyrosine-15…
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72465-plasmid-map-sequence-id-133904

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  2. pJK629

    Plasmid
    #72464
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to threonine, tyrosine…
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72464-plasmid-map-sequence-id-133903

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  3. pJK640

    Plasmid
    #72467
    Purpose
    Encodes Escherichia coli evgA
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72467-plasmid-map-sequence-id-134013

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  4. pJK541

    Plasmid
    #72447
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72447-plasmid-map-sequence-id-133869

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  5. pJK641

    Plasmid
    #72468
    Purpose
    Encodes Escherichia coli evgA (Plac noncoding)
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72468-plasmid-map-sequence-id-134015

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  6. pJK646

    Plasmid
    #72469
    Purpose
    Encodes Escherichia coli evgA (Plac coding)
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72469-plasmid-map-sequence-id-134016

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  7. pZC09 (pFA6a-TEV-6xGly-PrtA-His3MX)

    Plasmid
    #44073
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44073-plasmid-map-sequence-id-61686

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  8. pHH21-BNA-Luc

    Plasmid
    #187924
    Purpose
    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells
    Depositor
    Arindam Mondal
    Article
    Kedia et al Front Microbiol. 2022 May 25;13:868367.
    Insert
    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymease I Promoter
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187924-plasmid-map-sequence-id-373471

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  10. pAM5085

    Plasmid
    #85122
    Purpose
    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85122-plasmid-map-sequence-id-180108

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Showing: 631 - 640 of 8418 results