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Plasmid#197992PurposeExpressed sybody 25 as a C-terminal myc-His-tagged constructDepositorInsertSybody 25
UseTagsMyc-HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTP170 (pBAD33_CRISPR(Tn6900)-entry)
Plasmid#164263PurposeEntry vector for cloning user-defined spacers in the Tn6900 CRISPR array.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-RGG-GFP-RGG-mHP1alpha
Plasmid#181893PurposeSoluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mouse, Mustard Weed)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMB1-A-pBAD-dCas9
Plasmid#190132PurposeLevel 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRiDepositorInsertdCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pnGFP-Ultra
Plasmid#157923PurposePeroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)DepositorInsertpnGFP-Ultra, a high performance peroxynitrite sensor
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL1244
Plasmid#155138PurposeExpresses MlaFED operon in E. coliDepositorInsertmlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWA PAS-E BAD K-GAFm
Plasmid#39866DepositorInsertsiRFP protein PAS domain
iRFP protein GAF domain (mutated)
UseTagsE coil and K coilExpressionBacterialMutationF165Y, D232Y, W308RPromoterarabinose inducible and rhamnose inducibleAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHYBAD94
Plasmid#42305DepositorInsertNpuDnaEC6
UseTagsGB1-H6ExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMHBAD14
Plasmid#42304DepositorInsertNpuDnaE C-intein
UseTagsGB1-H6ExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N EBNA1
Plasmid#37954DepositorInsertEBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterTetracycline promoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEL2196
Plasmid#170400PurposeFor expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-717, replaced with 114-130)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-CRY2-OCRL
Plasmid#79565Purposeexpression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRLDepositorInsertOCRL (OCRL Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationaa 234_539PromoterCMVAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV cleavable His10ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2-OCRL(D523G)
Plasmid#79567Purposeexpression of CRY2PHR fused to phosphatase dead mutant of OCRLDepositorInsertOCRL (OCRL Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationaa 234_539 and phosphatase mutation, D523GPromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbMS2
Plasmid#171701PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbSARS
Plasmid#171703PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-FRISZ
Plasmid#176888PurposeFar-red fluorescent zinc indicator FRISZ in pTorPE vectorDepositorInsertFRISZ
UseTagsPeriplasmic expression TorA tag and Twin-Strep-ta…ExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only