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pAAV-CAG-H2B-GFP

Plasmid

#116869

Purpose

Nuclear localized GFP

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-EGFP-ATG13

Plasmid

#223797

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens, James Hurley

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 (ATG13 Human)

Use

Tags

GST-TEV-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2G

Plasmid

#159605

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2G

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Has Service

AAV1, AAV2, and AAV9

Insert

NIR-GECO2G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG R2pH-LAMP1-3xFLAG

Plasmid

#157940

Purpose

expresses ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Tags

3XFLAG, mCherry, and pHluorin

Expression

Mammalian

Mutation

Promoter

Chick beta actin pomoter

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Human, Synthetic)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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SLF063: Lenti-fCAG-jRGECO1a

Plasmid

#124775

Purpose

For general lentiviral or direct expression of cytosolic jRGECO1a

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

jRGECO1a

Use

Lentiviral

Tags

NES-6xHIS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only
pCAG-Oct3/4-IRES-Neo

Plasmid

#13461

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Oct3/4 (Pou5f1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

P31S

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-TetR_W43F-3xmNG

Plasmid

#174880

Purpose

PiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreen

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

TetR(W43F)-3xmNeonGreen

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/BFP Dest

Plasmid

#74282

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Tags

Expression

Mutation

Promoter

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-jGCaMP8m-WPRE

Plasmid

#179255

Purpose

AAV-mediated expression of GCaMP8m under the CAG promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-LplA(AILR,36)-NRX3b

Plasmid

#43919

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

AILR-LplA36-NRX3b (lplA Human, Synthetic)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Chicken beta actin

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Chronos-GFP

Plasmid

#99232

Purpose

AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BA.2 Spike

Plasmid

#185604

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

Insert

SARS-CoV-2 BA.2 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

July 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.1 Spike

Plasmid

#185452

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

Depositor

Marceline Côté

Article

Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

Insert

SARS-CoV-2 Spike BA.1 (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutations: A67V, Δ69-70, T…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pT/Caggs-NRAS G12V/D38A

Plasmid

#221073

Purpose

Expresses NRAS G12V/D38A in mammalian cells

Depositor

Hidenori Ichijo

Article

Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

Insert

NRAS (NRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G12V/D38A

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pR26-CAG-H2B-miRFP703

Plasmid

#112853

Purpose

Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

H2B-miRFP703

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-TurboID-3xFLAG

Plasmid

#186814

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

TurboID-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mPpp3cc-FLAG-6XHIS

Plasmid

#179160

Purpose

Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)

Use

Tags

6XHIS and FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.16-RBD

Plasmid

#213070

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.16-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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