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LV-AR-R599A-N600A

Plasmid

#171221

Purpose

Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.

Depositor

Steven Balk

Article

Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

Insert

AR-R599A-N600A (AR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

AR-R599A-N600A

Promoter

EF-1a

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1.shCDK8

Plasmid

#19760

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against CDK8 (CDK8 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
LV-AR-AAFF

Plasmid

#171218

Purpose

Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

Depositor

Steven Balk

Article

Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

Insert

AR-AAFF (AR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

AR-K580A-V581A

Promoter

EF-1a

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHR ErbB2-CD3ε-FusionRed

Plasmid

#188628

Purpose

Human ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

ERBB2 (ERBB2 Synthetic, Human)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB3-v1

Plasmid

#154893

Purpose

Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB3 (ERBB3 Human)

Use

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast ACSL4

Plasmid

#173164

Purpose

Retroviral vector to express sgRNA resistant human ACSL4

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)

Use

Retroviral

Tags

Expression

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast 3XFLAG-CHP1

Plasmid

#173166

Purpose

Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Calcineurin Like EF-Hand Protein 1 (CHP1 Human)

Use

Retroviral

Tags

3XFLAG

Expression

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB3-v2

Plasmid

#154894

Purpose

Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB3 (ERBB3 Human)

Use

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB2-v1

Plasmid

#154901

Purpose

Expresses ERBB2 fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB2 (ERBB2 Human)

Use

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV Cdk2ap1CAN-HA

Plasmid

#178030

Purpose

Retroviral vector for the purpose of overexpression in mammalian cell culture

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

First N-Terminal 27aa are deleted

Promoter

GAG

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast HA-GPAT4

Plasmid

#173168

Purpose

Retroviral vector to express sgRNA resistant HA tagged human GPAT4

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dCTNNA1

Plasmid

#176032

Purpose

A knock-out vector for dog CTNNA1 (alpha-1-catenin)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSB65 - pL0_tGFP-I (CDS1)

Plasmid

#123182

Purpose

Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites; codon-optimised for plants

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB2-v2

Plasmid

#154895

Purpose

Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB2 (ERBB2 Human)

Use

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_ZIKV_NS5_MTase

Plasmid

#213529

Purpose

Expresses the MTase domain of NS5 of ZIKV

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

ZIKA virus NS5 methyltransferase domain

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

MTase domain aa 1-265

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-wt

Plasmid

#184269

Purpose

Expresses shRNA resistant GFP-RepoMan

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Use

Tags

3xHA and GFP

Expression

Mammalian

Mutation

changed nucleotides 723-747, 1488-1509, 1524-1548…

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

Plasmid

#209197

Purpose

Mutagenesis of Faah

Depositor

Larry Zweifel

Article

Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

Insert

Faah (Faah Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

Plasmid

#210709

Purpose

This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

N55K in MCP

Promoter

U6

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV Cdk2ap1ΔN (MT2B2)-HA

Plasmid

#178031

Purpose

Retroviral vector for the purpose of overexpression in mammalian cell culture

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

First N-Terminal 27aa are deleted

Promoter

GAG

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dMET

Plasmid

#176031

Purpose

A knock-out vector for dog MET

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge