We narrowed to 3,081 results for: eed

1. pMIR-Report-Luc-KLF4-K6S

   Plasmid

   #62983

   Purpose

   Translational reporter for KLF4

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 partial sequence (KLF4 Human)
   

   Use

   Luciferase

   Tags

   firefly luciferase

   Expression

   Mutation

   Fragment 2086-2639 of KLF4

   Promoter

   CMV

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

   Plasmid

   #210709

   Purpose

   This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. M23: pHR-SFFVp-FUSN::mCherry::SspB

   Plasmid

   #122148

   Purpose

   N terminal domain of FUS (1-214) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FUS (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-214

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRK5-EGFP-Tau

   Plasmid

   #46904

   Purpose

   expresses EGFP tagged Tau in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   This WT htau construct contains human four-repeat…

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

   Plasmid

   #122668

   Purpose

   C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   HNRNPA1 (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-SspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 186-230

   Promoter

   SFFV

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. M31: pHR-SFFVp- TDP-43C::mCherry::SspB

   Plasmid

   #122669

   Purpose

   C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   TDP43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry-SspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 218-414

   Promoter

   SFFV

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-ERBB3-v2

   Plasmid

   #154894

   Purpose

   Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   ERBB3 (ERBB3 Human)
   

   Use

   Tags

   Venus fragment 2 (v2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST-ERBB2-v1

   Plasmid

   #154901

   Purpose

   Expresses ERBB2 fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   Venus fragment 1 (v1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSAM2_mCherry_Gata6

    Plasmid

    #72694

    Purpose

    Doxycyline regulated expression of Gata6 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMXS-IRES-Blast ACSL4

    Plasmid

    #173164

    Purpose

    Retroviral vector to express sgRNA resistant human ACSL4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXS-IRES-Blast HA-GPAT4

    Plasmid

    #173168

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST-ERBB2-v2

    Plasmid

    #154895

    Purpose

    Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    Venus fragment 2 (v2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe.puro.CDK8.flag

    Plasmid

    #19758

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hU6-pegRNA-EFS-mScarlet Template

    Plasmid

    #196037

    Purpose

    The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and EFS

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222497

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe.puro.CDK8.KD.flag

    Plasmid

    #19759

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D173A (kinase dead)

    Promoter

    Available Since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

18. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid

    #210710

    Purpose

    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    M11

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-EF1-IRES-NEO-Control

    Plasmid

    #80757

    Purpose

    Transposon Reporter

    Depositor

    Alex Kentsis

    Article

    Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

    Insert

    NeoR (neoR Synthetic)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    EF1-IRES from PB-EF1-IRES-NEO

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1.shCDK8

    Plasmid

    #19760

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    small hairpin RNA against CDK8 (CDK8 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only