We narrowed to 14,168 results for: eng
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Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorPublicationInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Ptet-nCas9-BE
Plasmid#195739PurposeExpress APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coliDepositorPublicationInsertTet repressor protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPtet(Tetracycline inducible protein)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS1106_Tier3(Lenti)
Plasmid#169661PurposeTier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)DepositorTypeEmpty backboneExpressionMammalianAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BYG
Plasmid#158126PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertmTagBFP
ExpressionYeastPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-pegRNA-H1-nick sgRNA-mCherry
Plasmid#176901PurposeExpresses prime editing guide RNA and guide RNA in mammalian cellsDepositorInsertsmCherry
gRNA scaffold
gRNA scaffold
UseAAVExpressionMammalianPromoterCAG, H1, and U6Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein promoter-less
Plasmid#118005PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, asPink
UseEscherichia coliMutationBioBrick sites removedAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
tsPurple chromoprotein promoter-less
Plasmid#118007PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, tsPurple
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilGFP chromoprotein promoter-less
Plasmid#118006PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilGFP
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
TagsMaltose binding protein and His tagExpressionBacterialPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
ExpressionBacterialPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS1026-Tier1-PhCMV-3xNLS-iRFP670
Plasmid#169532PurposeTier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).DepositorInsertPCMV-driven nucleus-localized far-red fluorescent protein iRFP670
ExpressionMammalianPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH233-Tier1-PmPGK1-eYFP
Plasmid#169522PurposeTier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).DepositorInsertPPGK-driven enhanced yellow fluorescent protein
ExpressionMammalianPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2404-Tier1-PhCMV-NeoR
Plasmid#169496PurposeTier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).DepositorInsertPCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II
ExpressionMammalianPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH227-Tier1-OTetO7-PCMVmin2-SEAP
Plasmid#169563PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).DepositorInserttetracycline-controlled SEAP production
ExpressionMammalianPromoterTetO7-PCMVminAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH232-Tier1-PmPGK1-mRuby2
Plasmid#169523PurposeTier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).DepositorInsertPPGK-driven monomeric red fluorescent protein mRuby2
ExpressionMammalianPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM545-Tier1-PhCMV-Citrine
Plasmid#169505PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).DepositorInsertPCMV-driven yellow fluorescent protein citrine
ExpressionMammalianPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM585-Tier1-PhCMV-Igk
Plasmid#169486PurposeTier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).DepositorAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only