We narrowed to 6,417 results for: nls

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AAV-Synapsin-mCherry-3XNLS-WPRE

Plasmid

#159951

Purpose

nucleus marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mCherry-3XNLS

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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phage UbiC NLS HA stdPCP stdGFP

Plasmid

#104099

Purpose

Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

stdPCP-stdGFP

Use

Lentiviral

Tags

N-terminal NLS, Two synonymous copies of GFP (C-t…

Expression

Mammalian

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-NeoR-CAG-EGFPnls-WPRE

Plasmid

#191692

Purpose

For knocking pCAG-EGFPnls into AAVS1 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

AAVS1 homology arms and pCAG-EGFPnls

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-LSL-nlsGFP-shRNA

Plasmid

#175584

Purpose

Conditional express shRNA with control of LoxP

Depositor

Christian Wolfrum

Article

Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

Type

Empty backbone

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EFS

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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phage UbiC NLS HA stdMCP Halo

Plasmid

#104098

Purpose

Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

stdMCP-Halo

Use

Lentiviral

Tags

C-terminal Halo-tag, N-terminal NLS, and Two syno…

Expression

Mammalian

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Tags

V5

Expression

Mammalian

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

Plasmid

#218793

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

NLSf-mScarletP2A-hLuc

Use

AAV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-MED1trunc-IDR-YFP-NLS

Plasmid

#145275

Purpose

Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MED1-IDR truncated (MED1 Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

Plasmid

#127362

Purpose

gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-Turbo-YFP-NLS-STOP-L2

Plasmid

#127361

Purpose

gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-3XBFP-2xNLS

Plasmid

#236371

Purpose

Overexpression of MCP-3XBFP-2xNLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

MCP-3XBFP-2xnls

Use

Lentiviral

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-NLS-CBGreen-FIRE-puro

Plasmid

#139195

Purpose

Luminescent reporter of ERK activity

Depositor

Matthew Lazzara

Article

Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

Insert

CBG99

Use

Luciferase and Retroviral

Tags

Fra1 PEST domain fragment (FIRE) and nuclear loca…

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

Plasmid

#133801

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA4

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pICH47811-pAtAct2::Cas9-nls::tSlATPase

Plasmid

#160221

Purpose

Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtACT2::Cas9-nls::tSlATPase

Use

Synthetic Biology; Moclo toolkit level 1 acceptor…

Expression

Plant

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

Plasmid

#133802

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA5

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRM055 His-TEV-Cas9 6xNLS 0C

Plasmid

#196246

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-DEVD-mCardinal-NLS

Plasmid

#164052

Purpose

Fluorescent reporter for caspase-3

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-mCardinal-NLS

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRM053 His-TEV-Cas9 triNLS 0C

Plasmid

#196244

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPyCAG-mKate2-3xNLS-IRES-Pac

Plasmid

#183618

Purpose

Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

Insert

mKate2-3xNLS

Tags

3xNLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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