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pAJM.719

Plasmid

#108520

Purpose

PTtg-YFP Reporter. Nar sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTtg-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGMCKq1-10M1-sspBmyc

Plasmid

#27069

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

tetR-PmyctetO-sspB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pCas9-J23109

Plasmid

#113150

Purpose

Plasmid constitutively expressing Cas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pRL_yEGFP_hc

Plasmid

#98746

Purpose

Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

Insert

Yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

synthetic

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBD21_037

Plasmid

#109154

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
iRFP720

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and pTet

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-sgControl_dual-MTS

Plasmid

#162765

Purpose

Expressing sgControl_dual-MTS

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgControl_dual-MTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-mRFP

Plasmid

#27058

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-mcherry rfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pTAPmyc-T2A-tagRFP

Plasmid

#140278

Purpose

Backbone for making a mammalian expression vector of the protein of interest

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-luxAB-ssrAec

Plasmid

#27062

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB-ssrAec

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2011

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3

Plasmid

#110532

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pIA1405

Plasmid

#172404

Purpose

Expression of SARS-COV-2 Nsp12 D218A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

D218A

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1420

Plasmid

#172406

Purpose

Expression of SARS-COV-2 Nsp12 R116A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

R116A

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1421

Plasmid

#172407

Purpose

Expression of SARS-COV-2 Nsp12 K50A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proD-B0032-E0051

Plasmid

#107241

Purpose

Expresses lacZa-GFP fusion from constitutive proD promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proD

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 K410N

Plasmid

#87280

Purpose

Encodes Hsh155 K410N for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

K410N

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMTG02-Amp-FRT

Plasmid

#128271

Purpose

Plasmid that produces the following fusion protein: TetR-LOV-RRRG(Degron)

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

TetR-LOV-RRRG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pPKC1 (JCE575)

Plasmid

#89773

Purpose

yeast expression of PKC1

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC1 (PKC1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

yeast PKC

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBD21_033

Plasmid

#109150

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
Sirius

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and pTet

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBD21_036

Plasmid

#109153

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
mKO2

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and pTet

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBD21_034

Plasmid

#109151

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
TagBFP

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and pTet

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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