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pDONR221-kcnk5b

Plasmid

#208989

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk5b (kcnk5b Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk6

Plasmid

#208990

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk6 (kcnk6 Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk7

Plasmid

#208991

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk7 (kcnk7 Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk9

Plasmid

#208992

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk9 (kcnk9 Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Without STOP codon

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk10a

Plasmid

#208993

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk10a (kcnk10a Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk10b

Plasmid

#208994

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk10b (kcnk10b Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk12

Plasmid

#208995

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk12 (kcnk12 Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk13a

Plasmid

#208996

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk13a (kcnk13a Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T1_gRNA

Plasmid

#199387

Purpose

pDEL111; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type I sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T2_gRNA

Plasmid

#199388

Purpose

pDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type II sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Zebrafish IntS6

Plasmid

#198410

Purpose

Expresses FLAG-tagged zebrafish IntS6

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

ints6 (ints6 Zebrafish)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Zebrafish IntS6L

Plasmid

#198411

Purpose

Expresses FLAG-tagged zebrafish IntS6L

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

ints6l (ints6l Zebrafish)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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mhc2dab:GFP-LT

Plasmid

#68996

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

GFP-LT

Expression

Mutation

Promoter

mhc2dab

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]

Plasmid

#198381

Purpose

Expression of SYPB::CRY2olig(535) in neurons of Danio rerio

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

UAS:sypb-egfp-cry2

Use

Tol2

Tags

EGFP

Expression

Mutation

D387A

Promoter

UAS

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb wnt3 promoter-Wnt3EGFP

Plasmid

#203793

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Wnt3-EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

Wnt3 (wnt3 Zebrafish)

Use

Zebrafish expression

Tags

mEGFP

Expression

Mutation

Promoter

4kb Wnt3 promoter

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_erbb2_gRNA171

Plasmid

#199385

Purpose

pDEL133; Gateway Destination vector for cell-type specific CRISPR encoding erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

erbb2 sgRNA 171

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-Alk3

Plasmid

#207614

Purpose

Zebrafish Alk3 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Vaucheria frigida, Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-BMPR2a

Plasmid

#207616

Purpose

Zebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

BMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Vaucheria frigida, Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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mhc2dab:mCherry

Plasmid

#68997

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

mhc2dab

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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