We narrowed to 2,550 results for: CCS

1. pLJD-KLF4-ER

   Plasmid

   #34596

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Retroviral

   Tags

   Myc epitope tag and estrogen receptor fusion

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pLenti PA-mCit-GW

   Plasmid

   #113456

   Purpose

   Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   ProteinA-mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

   Plasmid

   #90011

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Cdc25C S216A
   NBirA*-14-3-3e
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Ser 216 to Ala

   Promoter

   CMV

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TTmiRc2_BirA_CDKN1A

   Plasmid

   #136523

   Purpose

   Used as a donor vector to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

   Plasmid

   #62978

   Purpose

   Expresses myc-tagged mouse βTrCP residues 292-569

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   BTRC (Btrc Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   codes for residues 292-569

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMIR-Report-Luc-KLF4-K1S

   Plasmid

   #62979

   Purpose

   Translational reporter for KLF4

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 partial sequence (KLF4 Human)
   

   Use

   Luciferase

   Tags

   firefly luciferase

   Expression

   Mutation

   Fragment 1-446 of KLF4

   Promoter

   CMV

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLenti PA-NL

   Plasmid

   #113453

   Purpose

   Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-NanoLuc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PA-mCit

   Plasmid

   #113451

   Purpose

   Lentiviral ProteinA-mCitrine expression vector

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-mCitrine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBbC

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TTmiRC2_p53DD

   Plasmid

   #136520

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53DD (Trp53 Mouse)
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Expression

   Mutation

   This construct was created by PCR from the pBabe …

   Promoter

   Available since

   Oct. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGST-mBCL10

    Plasmid

    #33316

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    Bcl10 (Bcl10 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pTBL649 CHYRON2 integration construct

    Plasmid

    #126446

    Purpose

    To integrate the CHYRON2 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON2 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIR-Report-RASA1-3'UTR

    Plasmid

    #62575

    Purpose

    Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    RASA1 (RASA1 Human)
    

    Use

    Luciferase

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET-22HT-MSI1(7-192)R53E/R59E/R61E

    Plasmid

    #60355

    Purpose

    R53E R59E R61E triple mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E, R59E and R61E mutations; contains amino aci…

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

14. XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

    Plasmid

    #233388

    Purpose

    PiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    IL2Rb(ecto+TM)-ADAR2dd
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

    Plasmid

    #231206

    Purpose

    Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L147I, R148H, I203V, L290V

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2

    Plasmid

    #231208

    Purpose

    Single transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L80F, R81D, L85V, I86V, I92F

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CA-0176 CMVTO-mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra

    Plasmid

    #231202

    Purpose

    CageIL-10-mono (mouse renin cut-site)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N36I, N110I, K117N, F129L

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CA244 CMVTO_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

    Plasmid

    #231205

    Purpose

    Single transcript cageIL-10 (mRens) + ER-renin (H2M)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L147I, R148H, I203V, L290V

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CA-0266 CMVTO-IVT-UTR-mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin (FLP-IN)

    Plasmid

    #231209

    Purpose

    Single transcript mRNA cageIL-2 (mRens) + ER-renin (H2M)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    UTR-mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin (IL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L80F, R81D, L85V, I86V, I92F

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CA-0252 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens-cleaved

    Plasmid

    #231215

    Purpose

    Cleaved-myc-IL-10

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    c-myc-mono-hIL10 (R5A11M)-rens-cleaved (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N36I, N110I, K117N, F129L

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge