We narrowed to 6,084 results for: nls

1. pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106352

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

   Plasmid

   #81206

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. ubc-nls-pcp-5xSunTag (SRv1)

   Plasmid

   #185797

   Purpose

   pcp-5xSunTag component for SunRISER SRv1

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   pcp-5xSunTag
   

   Use

   Tags

   FactorXa site, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-AICD-NLS-IRES-hrGFP

   Plasmid

   #107543

   Purpose

   AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

   Depositor

   Hélène Marie

   Article

   Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

   Insert

   AICD-NLS (APP Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

   Plasmid

   #115140

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

   Depositor

   Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

   Plasmid

   #115138

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

   Depositor

   Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA2 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Construct 7 - CMVp-Cas9-3xNLS-HSVpA

   Plasmid

   #81247

   Purpose

   Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

   Plasmid

   #49313

   Purpose

   Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Insert

   nls-mCherry-Flag
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Crimson/P2A-NLS/HA-HumBeta (pSLIK2)

   Plasmid

   #113857

   Purpose

   For Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase gene

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   Humanized Beta
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Expression

   Mutation

   "humanized" Beta gene

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106355

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    MYOD1 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xNLS-NLP-cMyc-cMyc AspCas12a

    Plasmid

    #182122

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80065

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJFRC7-RFPnls-PQRv2-mCD8:GFP

    Plasmid

    #73974

    Purpose

    Protein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.

    Depositor

    Brian Chen

    Article

    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

    Inserts

    sfGFP
    RFP
    

    Use

    Tags

    mCD8 and nls

    Expression

    Insect

    Mutation

    Promoter

    20XUAS-HS

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3xFLAG-CMV-Drosha (NLS-P426)

    Plasmid

    #171538

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    an SV40 NLS sequence (PKKKRKVGI) insertion into t…

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80061

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

    Plasmid

    #134334

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA17_Sga
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

    Plasmid

    #134335

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA18_Sma
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

    Plasmid

    #134336

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA19_Ssim
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)

    Plasmid

    #134338

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA18_Sga
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

    Plasmid

    #134339

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA19_Spse
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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