We narrowed to 18,759 results for: nls

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pCAGG>nls-hCas9-nls-GFP

Plasmid

#99141

Purpose

Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

Depositor

Marianne Bronner

Publication

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9-GFP

Use

CRISPR

Tags

GFP

Promoter

Chicken beta actin (CAG)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-tdTomato-CNA35

Plasmid

#61606

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

Depositor

Maarten Merkx

Publication

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

tdTomato and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

Plasmid

#219711

Purpose

Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.

Depositor

Jennifer Nemhauser

Insert

GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4

Use

Synthetic Biology

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWMD23

Plasmid

#140006

Purpose

Expression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Inserts

ORF1 Shuffle 1 NLS
Pong TPase LA

Expression

Plant

Mutation

NES sequence mutated L418A, L420A and Ping N-term…

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR

Plasmid

#67932

Purpose

Translational reporter - Lox 5'UTR

Depositor

Ron Vale

Publication

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

sfGFP-NLS-P2A-NLS-mCherry (LOX Human)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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PABE-1

Plasmid

#115622

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

NLS-wtTadA-TadA7.10-nCas9-NLS

Use

CRISPR

Expression

Plant

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFUGW NLS-FlpO

Plasmid

#183448

Purpose

Lentiviral FlpO vector

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-FlpO

Use

Lentiviral

Expression

Mammalian

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR-NLS

Plasmid

#118467

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sapphireAKAR-NLS

Tags

Nuclear localization signal (NLS)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Publication

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls

Plasmid

#99138

Purpose

Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

Depositor

Marianne Bronner

Publication

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized Cas9

Use

CRISPR

Promoter

Chicken beta actin (CAG)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pH-A3A-PBE

Plasmid

#119774

Purpose

Conversion of C to T in rice plants transformation

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP

Plasmid

#64111

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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JAB3477

Plasmid

#190416

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XPhlFO-3XAmtRO::TetR-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Publication

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

6XPhlFO-3XAmtRO::TetR-NLS-19St

Expression

Plant

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2964

Plasmid

#190398

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XAmtRO-2XPhlFO::TetR-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Publication

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

6XAmtRO-2XPhlFO::TetR-NLS-19St

Expression

Plant

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGGB-OCS terminator-SunTag-HA-dCas9-NLS

Plasmid

#246798

Purpose

A GreenGate entry vector containing NLS-dCas9-HA-SunTag fused gene followed by a OCS terminator

Depositor

Danhua Jiang

Insert

NLS-dCas9-HA-SunTag-OCS terminator

Use

Greengate cloning entry vector

Available since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMEH303 NLS dTomato 6x high affinity MS2 loops

Plasmid

#168215

Purpose

Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

Depositor

Joshua Leonard

Publication

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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LEXY TR (with KRAB domain) (pDN163)

Plasmid

#72662

Purpose

LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repression

Depositor

Barbara Di Ventura, Roland Eils

Publication

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MTK3a_003

Plasmid

#123756

Purpose

Encodes Nuclear import sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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A3A-PBE

Plasmid

#119768

Purpose

Conversion of C to T in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A and E945K in wild type …

Promoter

maize Ubiquitin-1

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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